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Preservation of Eukaryotic Cells using Reversible Pore Formation

Detailed Technology Description: This technology is a method for stabilizing eukaryoticcells, particularly mammalian and human cells, in order to introduce otherwisemembrane impermeable compounds that are helpful for stabilizing the cellsduring drying, chilling, freezing, freeze-drying, or cryopreservation.

Countries: United States

Application No.: 7,314,755

*Abstract: The cells are exposed to extracellular ATP (adenosine5'-triphosphate) in concentrations that are sufficient to open pores in theplasma membrane. The otherwise membrane-impermeable compounds that aid insurvivorship of cells are introduced, and after a brief time the pores areclosed. Once the stabilizing compound has been introduced, the cells may bestably preserved and no antigenic compounds need to be introduced.

*IP Issue Date: Jan 1, 2008

*IP Type: Utility

*Licensing: Robert J. BrownAssistant DirectorTel: 225-615-8916Email: rjbrown@lsu.edu

Country/Region: USA

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