Bioactivation and Surface Properties Modulation of Inorganic Nanoparticles
Allows for microparticles and nanoparticles to be used in biological systems in which they would ordinarily not be effective (i.e., semiconductor nanocrystals).
Permits the use of inorganic, organic, metallic, and semiconducting nanoparticles in biological systems. Potentially useful for drug discovery, medical imaging, therapeutic reagents, self-assembly of nano-machines by molecular recognition, light-controlled enzymes and catalysts, and bio-mimetic materials.
The invention allows inorganic particles to be used in biological systems. By attaching specialized peptides to the surface of the particle, this invention allows these inorganic particles to take on biologically important functions. It provides water solubility and bioactivity to inorganic, metallic, and semiconducting nanoparticles as well as organic particles. The peptides also have a functional end that allows for adjusting the nanoparticles' targeting capabilities by way of interchangeable sequences. Thus, in line with the example above, this invention would permit the use of semiconductor nanocrystals as fluorescent labels in biological systems.
Patent Number: US7998923B2
Application Number: US2003513567A
Inventor: Pinaud, Fabien | King, David | Weiss, Shimon
Priority Date: 7 May 2002
Priority Number: US7998923B2
Application Date: 3 Nov 2004
Publication Date: 16 Aug 2011
IPC Current: A61K003800 | A61K004748 | A61K005100
US Class: 5140011 | 42400169 | 530300
Assignee Applicant: The Regents of the University of California
Title: Bioactivation of particles
Usefulness: Bioactivation of particles
Summary: (II) is useful in producing a bioactivated particle, preferably bioactivated quantum dot that is soluble in an aqueous medium, which involves providing a particle that includes a surface or providing a quantum dot, treating the surface of the particle or the quantum dot with a sufficient amount of (II) to make the bioactivated nanoparticle or bioactivated quantum dot soluble in the aqueous medium (claimed). (I) (I) is useful in targeting, imaging and therapeutical applications. The bioactivation peptide of (I) is useful for semiconductor nanocrystals (NCs) fluorescent probe targeting, targeting of particles to tumors for X-ray medical imaging, for providing molecular recognition capabilities to the particles, and also in peptide library screening/phage display, in vivo / in vitro drug screening, in vivo / in vitro multicolor assays for application of quantum dots in atomic force microscopy (as probes), and assays based on peptide/peptide interaction, protease responsive sequence, peptide/DNA interaction, peptide/RNA interaction, molecular dynamic of antibody/antigen interactions by single molecule detection/quenching or single molecule fluorescence resonance energy transfer (FRET), crystallography two-dimensional, three-dimensional arrays for protein structure analysis, solid phase hybridization assay using quantum dots as a support, enzyme kinetic assays and peptide-nucleic acid (PNA) technology. The bioactivation peptides are useful in self-assembly of organic-inorganic nanostructure hybrids by molecular recognition, and in making interfacing enzymes, biocatalysts and other proteins/RNA catalysts to (II) nanoparticles to produce nanomachines/molecular machines that are activated by light and/or charge.
Novelty: Bioactivated particles useful in imaging and targeting applications, comprising a particle with a surface, and a bioactivation peptide comprising a surface recognition portion and functional portions, attached to the surface of the particle
Biomedical
Medical Imaging
7998923
Background Related Materials Additional Technologies by these Inventors Tech ID/UC Case 21728/2002-243-0 Related Cases 2002-243-0
Semiconductor nanocrystals (also known as quantum dots) provide a very real solution to the limitations of organic dye molecules. Varying the size of the nanocrystals allows a tuning of the emission wavelength without changing the absorption characteristics. Further, they emit a strong fluorescent signal that remains stable for a much longer period of time. However, these semiconductor nanocrystals are highly hydrophobic particles. As a result, to have any significant biological application, surface chemistry is necessary to make the particle biocompatible and soluble in aqueous environments.
USA
