Regulatable Gene Expression In Bacterial Cells Using A 2-methylcitrate-resposive Prpbcde Promoter
The combined gains in inducibility and yield present many opportunities for the propionate inducible expression system in laboratory and industrial applications.
Expression of recombinant protein in E. coli is highly-regulated by the inducer.? High level of expression can be achieved in the presence of inducer (maximal induction 1500 fold).? The inducer propionate is non-toxic and inexpensive; therefore allowing for large-scale industrial production. In contrast, some commonly used inducers such as IPTG are expensive and toxic to cells. ? Uniform expression: cells are uniformly induced to express recombinant protein in the presence of inducer whereas in many other inducible systems, the induction level varies among individual cells in the whole population. ? The basal level of expression in the absence of inducer is negligible.
None
Patent Number: US8178338B2
Application Number: US2007993419A
Inventor: Keasling, Jay D. | Lee, Sung Kuk
Priority Date: 1 Jul 2005
Priority Number: US8178338B2
Application Date: 25 Nov 2008
Publication Date: 15 May 2012
IPC Current: C12N001500
US Class: 43525233 | 4353201 | 5360241
Assignee Applicant: The Regents of the University of California
Title: Inducible expression vectors and methods of use thereof
Usefulness: Inducible expression vectors and methods of use thereof
Summary: The nucleic acid is useful in producing a gene product in a host cell.
Novelty: New nucleic acid comprising a promoter that is inducible by a transcriptional activator protein and a nucleotide sequence that encodes the transcriptional activator protein, useful in producing a gene product in a host cell
Biomedical
DNA/Gene Engineering
8178338
Tech ID/UC Case 17542/2005-107-0 Related Cases 2005-107-0
USA
