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Regulatable Gene Expression In Bacterial Cells Using A 2-methylcitrate-resposive Prpbcde Promoter

技术优势: The combined gains in inducibility and yield present many opportunities for the propionate inducible expression system in laboratory and industrial applications.

技术应用: Expression of recombinant protein in E. coli is highly-regulated by the inducer.? High level of expression can be achieved in the presence of inducer (maximal induction 1500 fold).? The inducer propionate is non-toxic and inexpensive; therefore allowing for large-scale industrial production. In contrast, some commonly used inducers such as IPTG are expensive and toxic to cells. ? Uniform expression: cells are uniformly induced to express recombinant protein in the presence of inducer whereas in many other inducible systems, the induction level varies among individual cells in the whole population. ? The basal level of expression in the absence of inducer is negligible.

*Abstract: Inducible expression systems are essential molecular tools for production of recombinant proteins. Although there are currently a number of protein expression systems available, there is a need to develop new systems that allow for tighter regulation and higher yield. This invention provides compositions and methods for an inducible protein expression system in E. coli based on a novel propionate inducible promoter.

*Principal Investigation: Name: Jay Keasling
Department:

Name: Sung Lee
Department:

附加资料: Patent Number: US8178338B2
Application Number: US2007993419A
Inventor: Keasling, Jay D. | Lee, Sung Kuk
Priority Date: 1 Jul 2005
Priority Number: US8178338B2
Application Date: 25 Nov 2008
Publication Date: 15 May 2012
IPC Current: C12N001500
US Class: 43525233 | 4353201 | 5360241
Assignee Applicant: The Regents of the University of California
Title: Inducible expression vectors and methods of use thereof
Usefulness: Inducible expression vectors and methods of use thereof
Summary: The nucleic acid is useful in producing a gene product in a host cell.
Novelty: New nucleic acid comprising a promoter that is inducible by a transcriptional activator protein and a nucleotide sequence that encodes the transcriptional activator protein, useful in producing a gene product in a host cell

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