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Regulatable Gene Expression In Bacterial Cells Using A 2-methylcitrate-resposive Prpbcde Promoter

Technology Benefits

The combined gains in inducibility and yield present many opportunities for the propionate inducible expression system in laboratory and industrial applications.

Technology Application

Expression of recombinant protein in E. coli is highly-regulated by the inducer.? High level of expression can be achieved in the presence of inducer (maximal induction 1500 fold).? The inducer propionate is non-toxic and inexpensive; therefore allowing for large-scale industrial production. In contrast, some commonly used inducers such as IPTG are expensive and toxic to cells. ? Uniform expression: cells are uniformly induced to express recombinant protein in the presence of inducer whereas in many other inducible systems, the induction level varies among individual cells in the whole population. ? The basal level of expression in the absence of inducer is negligible.

Detailed Technology Description

None

Supplementary Information

Patent Number: US8178338B2
Application Number: US2007993419A
Inventor: Keasling, Jay D. | Lee, Sung Kuk
Priority Date: 1 Jul 2005
Priority Number: US8178338B2
Application Date: 25 Nov 2008
Publication Date: 15 May 2012
IPC Current: C12N001500
US Class: 43525233 | 4353201 | 5360241
Assignee Applicant: The Regents of the University of California
Title: Inducible expression vectors and methods of use thereof
Usefulness: Inducible expression vectors and methods of use thereof
Summary: The nucleic acid is useful in producing a gene product in a host cell.
Novelty: New nucleic acid comprising a promoter that is inducible by a transcriptional activator protein and a nucleotide sequence that encodes the transcriptional activator protein, useful in producing a gene product in a host cell

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

8178338

Others

Tech ID/UC Case

17542/2005-107-0

Related Cases

2005-107-0

*Abstract

Inducible expression systems are essential molecular tools for production of recombinant proteins. Although there are currently a number of protein expression systems available, there is a need to develop new systems that allow for tighter regulation and higher yield. This invention provides compositions and methods for an inducible protein expression system in E. coli based on a novel propionate inducible promoter.

*IP Issue Date

May 15, 2012

*Principal Investigator

Name: Jay Keasling

Department:

Name: Sung Lee

Department:

Country/Region

USA