Overexpression of Histone Increases Plant Transformation by Agrobacterium
- Summary
- Purdue University researchers have found that a particular histone H2A variant of Arabidopsis is involved in Agrobacterium T-DNA integration into the Arabidopsis genome. A plant carrying a mutation of this gene cannot integrate T-DNA; however, the wild-type allele can complement this mutation. In addition, overexpression of the histone H2A gene in wild-type Arabidopsis increases the efficiency of Agrobacterium mediated transformation. Because the histone H2A gene can reside on the incoming T-DNA and be expressed in the recipient cell, researchers speculate that this effect will improve transformation rates in recalcitrant plants.
- Technology Benefits
- Stable Agrobacterium transformation of recalcitrant plants Unique solution to plant transformation problems
- Technology Application
- Agriculture R&D
- Detailed Technology Description
- Stanton GelvinPurdue Biological Sciences
- Countries
- United States
- Application No.
- 7,122,716
- *Abstract
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- *Background
- Agrobacterium has proven to be a useful tool in the transformation of some, but not all, plants. One problem among recalcitrant plants is the failure of the T-DNA to become integrated into the host genome. As a result, transformation occurs, but it is transient.
- *IP Issue Date
- Oct 17, 2006
- *IP Type
- Utility
- *Stage of Development
- Prototype testing validated
- *Web Links
- Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipStanton GelvinPurdue Biological Sciences
- Country/Region
- USA
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