Overexpression of Histone Increases Plant Transformation by Agrobacterium
Purdue University researchers have found that a particular histone H2A variant of Arabidopsis is involved in Agrobacterium T-DNA integration into the Arabidopsis genome. A plant carrying a mutation of this gene cannot integrate T-DNA; however, the wild-type allele can complement this mutation. In addition, overexpression of the histone H2A gene in wild-type Arabidopsis increases the efficiency of Agrobacterium mediated transformation. Because the histone H2A gene can reside on the incoming T-DNA and be expressed in the recipient cell, researchers speculate that this effect will improve transformation rates in recalcitrant plants.
Stable Agrobacterium transformation of recalcitrant plants Unique solution to plant transformation problems
Agriculture R&D
Stanton GelvinPurdue Biological Sciences
United States
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