Group Specific Internal Standard Technology and Labeling Strategy
- Summary
- Researchers at Purdue University have dramatically improved precision by developing a novel post-biosynthetic encoding concept using derived samples as internal standards. This cost-effective global derivation approach enables absolute quantification of known compounds and relative quantification of unknown compounds in most cases using a single LC-MS run.
- Technology Benefits
- Aniline based derivation agent labels most of the metabolites or other small moleculesAbsolute quantification of known compoundsRelative quantification of unknown compounds, no standards are necessaryMost of these compounds can be analyzed in single LC-MS run
- Technology Application
- Encoding Technology
- Detailed Technology Description
- Bindley Bioscience Center
- Countries
- United States
- Application No.
- 8,628,977
- *Abstract
-
- *Background
- Precision quantification of metabolite concentrations is important to identify relationships within biological systems. Traditional ways to quantify metabolites include gas and liquid chromatography for separation and mass spectrometry for identification and quantification. A major limitation to precision quantification is ion suppression.
- *IP Issue Date
- Jan 14, 2014
- *IP Type
- Utility
- *Stage of Development
- Proof of Concept
- *Web Links
- Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipBindley Bioscience Center
- Country/Region
- USA
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