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Group Specific Internal Standard Technology and Labeling Strategy

总结
Researchers at Purdue University have dramatically improved precision by developing a novel post-biosynthetic encoding concept using derived samples as internal standards. This cost-effective global derivation approach enables absolute quantification of known compounds and relative quantification of unknown compounds in most cases using a single LC-MS run.
技术优势
Aniline based derivation agent labels most of the metabolites or other small moleculesAbsolute quantification of known compoundsRelative quantification of unknown compounds, no standards are necessaryMost of these compounds can be analyzed in single LC-MS run
技术应用
Encoding Technology
详细技术说明
Bindley Bioscience Center
*Abstract

*Background
Precision quantification of metabolite concentrations is important to identify relationships within biological systems. Traditional ways to quantify metabolites include gas and liquid chromatography for separation and mass spectrometry for identification and quantification. A major limitation to precision quantification is ion suppression.
*IP Issue Date
Jan 14, 2014
*IP Type
Utility
*Stage of Development
Proof of Concept
*Web Links
Purdue Office of Technology CommercializationPurdueInnovation and EntrepreneurshipBindley Bioscience Center
国家
United States
申请号码
8,628,977
国家/地区
美国

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