Method for Engineering Functional 3-Dimensional Kidney Tissue
Scientists at the University of California have developed a novel in vitro method that utilizes elements of kidney primordia, the Wolffian duct (WD) and metanephric mesenchyme (MM), to engineer in vitro kidney-like tissue containing functional tubular transporters and glomeruli with apparent early vascularization. This method provides a potential strategy for engineering a 3D vascularized kidney-like tissue from cells in vitro, if a tubular structure can be formed from adult, amniotic, embryonic stem cells or other cell types. Moreover, this in vitro stepwise approach provides the potential for introduction of immunomodulatory or other genes.
Patent Number: US20140011278A1
Application Number: US13911755A
Inventor: Nigam, Sanjay Kumar | Rosines, Eran
Priority Date: 4 Jun 2007
Priority Number: US20140011278A1
Application Date: 6 Jun 2013
Publication Date: 9 Jan 2014
IPC Current: C12N0005071
US Class: 435377
Assignee Applicant: The Regents of the University of California
Title: METHODS OF TISSUE GENERATION AND TISSUE ENGINEERED COMPOSITIONS
Usefulness: METHODS OF TISSUE GENERATION AND TISSUE ENGINEERED COMPOSITIONS
Summary: The methods are useful for generating a tubular tissue structure; in vitro culturing and propagating UB, WD bud, or ureteric and WD bud tissue; growing renal tubule cells in vitro; and for forming rudimentary kidney tissue (all claimed). The method can also be used for treating renal failure.
Novelty: Generating tubular tissue structure by contacting stem cell, ureteric bud (UB) cell, or Wolffian duct (WD) cell with an active agent, contacting the cells with a branching agent, and combining UB tissue with metenephric mesenchyme (MM)
Biomedical
Bioengineering
8460929
Publication Nigam, S. K., Staged in vitro reconstitution and implantation of engineered rat kidney tissue, PNAS 2007 104: 20938-20943 Additional Technologies by these Inventors Tech ID/UC Case 22717/2007-300-0 Related Cases 2007-300-0
USA
