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Method for Engineering Functional 3-Dimensional Kidney Tissue

詳細技術說明

Scientists at the University of California have developed a novel in vitro method that utilizes elements of kidney primordia, the Wolffian duct (WD) and metanephric mesenchyme (MM), to engineer in vitro kidney-like tissue containing functional tubular transporters and glomeruli with apparent early vascularization.  This method provides a potential strategy for engineering a 3D vascularized kidney-like tissue from cells in vitro, if a tubular structure can be formed from adult, amniotic, embryonic stem cells or other cell types.  Moreover, this in vitro stepwise approach provides the potential for introduction of immunomodulatory or other genes.

*Abstract

End stage renal disease (ESRD) affects approximately 400,000 individuals in the United States alone, and this number continues to increase rapidly.  While dialysis provides life-saving treatment to patients with ESRD and/or can bridge the time between kidney failure and the receipt of a transplant, only 78% of patients are reported to survive the first year of dialysis and the 10-year survival rate is only 9%.  With over 60,000 people waiting for kidney transplants, the improvement in short-term allograft survival has shifted attention to the two major remaining challenges in kidney transplantation: the shortage of organs and the lack of improvement in the rate of allograft failure after the first post-transplant year.  To address the shortage of donor organs, a variety of tissue-engineering strategies are being pursued, including the extracorporeal renal tubule assist device, the transplantation of renal primordia, the injection of stem-like cells into diseased kidneys and the in vitro engineering of kidneys.  The engineering of a kidney-like tissue from cells with appropriate 3D spatial relationships of nephrons has yet to be achieved.

*IP Issue Date

Jun 4, 2013

*Principal Investigation

Name: Sanjay Nigam

Department:

Name: Eran Rosines

Department:

附加資料

Patent Number: US20140011278A1
Application Number: US13911755A
Inventor: Nigam, Sanjay Kumar | Rosines, Eran
Priority Date: 4 Jun 2007
Priority Number: US20140011278A1
Application Date: 6 Jun 2013
Publication Date: 9 Jan 2014
IPC Current: C12N0005071
US Class: 435377
Assignee Applicant: The Regents of the University of California
Title: METHODS OF TISSUE GENERATION AND TISSUE ENGINEERED COMPOSITIONS
Usefulness: METHODS OF TISSUE GENERATION AND TISSUE ENGINEERED COMPOSITIONS
Summary: The methods are useful for generating a tubular tissue structure; in vitro culturing and propagating UB, WD bud, or ureteric and WD bud tissue; growing renal tubule cells in vitro; and for forming rudimentary kidney tissue (all claimed). The method can also be used for treating renal failure.
Novelty: Generating tubular tissue structure by contacting stem cell, ureteric bud (UB) cell, or Wolffian duct (WD) cell with an active agent, contacting the cells with a branching agent, and combining UB tissue with metenephric mesenchyme (MM)

主要類別

生物醫學

細分類別

生物工程

申請號碼

8460929

其他

Publication

Nigam, S. K., Staged in vitro reconstitution and implantation of engineered rat kidney tissue, PNAS 2007 104: 20938-20943

Additional Technologies by these Inventors

• Methods of Tissue Repair, Regeneration, and Tissue Engineered Compositions
• A Method for Rapid Generation of Many Different Branched Epithelial Proto-Organs

Tech ID/UC Case

22717/2007-300-0

Related Cases

2007-300-0

國家/地區

美國