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Cell culture method enables distinct labeling of newly synthesized protein with minimal disruption

Summary

Wei Min, Ph.D.

Technology Benefits

Can clearly and distinctly image both total protein and deuterated (nascent) proteinDoes not require fluorescent or radio-isotope labelingNot prone to bleaching; signal persists for long durationsImaging can be done with live or fixed cellsLittle overlap with other imaging methods, allowing researchers to use other imaging techniques simultaneouslyPatent information:Patent PendingTech Ventures Reference: IR CU13332

Technology Application

Modified cell growth medium for incorporation of deuterated amino acidsMinimally disruptive labeling method for Raman labeling in live cellsProvides precise temporal and spatial information on the changing proteome during various processes

Detailed Technology Description

Wei Min, Ph.D.

Country/Region

USA

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