Cell culture method enables distinct labeling of newly synthesized protein with minimal disruption
- 总结
- Wei Min, Ph.D.
- 技术优势
- Can clearly and distinctly image both total protein and deuterated (nascent) proteinDoes not require fluorescent or radio-isotope labelingNot prone to bleaching; signal persists for long durationsImaging can be done with live or fixed cellsLittle overlap with other imaging methods, allowing researchers to use other imaging techniques simultaneouslyPatent information:Patent PendingTech Ventures Reference: IR CU13332
- 技术应用
- Modified cell growth medium for incorporation of deuterated amino acidsMinimally disruptive labeling method for Raman labeling in live cellsProvides precise temporal and spatial information on the changing proteome during various processes
- 详细技术说明
- Wei Min, Ph.D.
- *Abstract
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None
- *Inquiry
- Beth KaudererColumbia Technology VenturesTel: (212) 854-8444Email: TechTransfer@columbia.edu
- *IR
- CU13332
- *Principal Investigation
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- *Publications
- L. Wei et al. Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy. PNAS, Vol. 110, Issue 28, July 2013, pp.11226-11231.
- 国家/地区
- 美国
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