Rapid Genetic Screening Method
A Rapid And Sensitive Detection Method Capable Of Reliably Distinguishing Fragments Identical To A Defined Sequence From Fragments Containing A Single Base Pair Mismatch Relative To The Defined Sequence. The Method Employs A Peptide Analog Antisense Probe, Such As A Peptide Nucleic Acid (Pna) Probe, Which Hybridizes With Dna To Forms Complexes Having Higher Thermal Stability, But Greater Sensitivity To Base Pair Mismatches, Than Corresponding Dna/Dna Complexes. The Method May Be Used In A Number Of Applications, Including Genetic Screening And Hybridization Sequencing Applications.
Patent Number: US6020126A
Application Number: US1997821524A
Inventor: Carlsson, Christina | Dulay, Maria T. | Jonsson, Mats | Nielson, Peter | Norden, Bengt | Tsui, Lap Chee | Zare, Richard | Zielinski, Julian
Priority Date: 21 Mar 1996
Priority Number: US6020126A
Application Date: 21 Mar 1997
Publication Date: 1 Feb 2000
IPC Current: C07H002100 | C12Q000168
US Class: 43500614 | 435006 | 204451 | 43500616 | 5360221
Assignee Applicant: HSC Reasearch and Development Limited Partnership,Toronto | The Board of Trustees of The Leland Stamford Junior University,Palo Alto
Title: Rapid genetic screening method
Usefulness: Rapid genetic screening method
Summary: The method may be used to screen for genetic diseases such as sickle-cell disease, Duchenne muscular dystrophy, cystic fibrosis and Tay Sachs.
Novelty: Detecting single base pair mismatches in target nucleic acid by using peptide nucleic acid probes, useful for screening genetic diseases such as sickle-cell disease, Duchenne muscular dystrophy, cystic fibrosis and Tay Sachs
Biomedical
DNA/Gene Engineering
Hong Kong
