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Trypanosoma cruzi CRISPR/Cas9 System Plasmid, tdTomato/pTREX-b

Detailed Technology Description

Applications This plasmid possesses cytoplasmic red fluorescence andblasticidin resistance and can be used to transfect Trypanosoma cruziepimastigotes expressing Cas9 (green fluorescent and neomycin resistant) togenerated CRISPR-ablated, red/green fluorescent parasites.Trypanosoma cruzi CRISPR/Cas9 System plasmids allow for theamplification of a specific sgRNA sequence or express cas9 to generateCRISPR-ablated, red/green fluorescent parasites.Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.

*Abstract

Reagent Description

 

Gene/Insert Name: tdTomato Insert Size (bp): 1430 Species:   Synthetic Fusion Proteins/Tags: Vector Backbone and Size (bp): pTREX-b, backbone size without insert: ~5868bp              Cloning Site 5’: XbaI Cloning Site 3’: HindIII Antibiotic Resistance: Ampicillin High or Low Copy:   High Grow in Standard E. coli @ 37oC? DH5alpha; 37°C Selectable Markers: Blasticidin Recommended Storage Temperature:   -20oC

References

 

LanderN, Li ZH, Niyogi S, Docampo R. CRISPR/Cas9-Induced Disruption of ParaflagellarRod Protein 1 and 2 Genes in Trypanosomacruzi Reveals Their Role in Flagellar Attachment. mBIO. 2015.6(4): e01012-15.

Country/Region

USA