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Trypanosoma cruzi CRISPR/Cas9 System Plasmid, tdTomato/pTREX-b


详细技术说明

Applications This plasmid possesses cytoplasmic red fluorescence andblasticidin resistance and can be used to transfect Trypanosoma cruziepimastigotes expressing Cas9 (green fluorescent and neomycin resistant) togenerated CRISPR-ablated, red/green fluorescent parasites.Trypanosoma cruzi CRISPR/Cas9 System plasmids allow for theamplification of a specific sgRNA sequence or express cas9 to generateCRISPR-ablated, red/green fluorescent parasites.Trypanosomacruziis the agent of Chagas disease, which affects millions of people worldwide.Vaccines to prevent this disease are not available, and drug treatments are notcompletely effective. The study of the biology of this parasite through geneticapproaches will make possible the development of new preventive or treatmentoptions.


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美国

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