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Plasmonic Dark Field Microscopy

Technology Benefits

PDF microscopy promises to deliver unprecedented performance in terms of spatial resolution, optical contrast, and device miniaturization and versatility at low cost.

Detailed Technology Description

UC San Diego researchers have developed a new optical imaging technique referred to as “plasmonic dark field” (PDF) microscopy. Instead of the lenses, light stops, and mirrors of conventional dark field microscopy, PDF microscopy uses a “plasmonic condenser” (PC) to guide the sample illumination angle. In one embodiment, the PC is essentially comprised of a metallic structure that converts light into surface plasmons. Since surface plasmons exist only at the metal surface, they appear completely dark in the far field. Only when an object is brought in the near field of the metal surface will light couple into the far field to form a perfect dark field image. The invention also includes active plasmonic couplers that use media, e.g., luminescent materials that provide mechanisms for exciting surface plasmons in a metal layer. The PC devices can be fabricated using well-established processes. Proof-of-concept for the invention has been achieved by numerical simulations and experiments. In particular, a chip-scale integrated multilayered fluorescent active PC has been used to demonstrate the new imaging technique.

Supplementary Information

Inventor: Arienti, Kristen L. | Breitenbucher, James G. | Buzard, Daniel J. | Edwards, James P. | Hack, Michael D. | Lee-Dutra, Alice | Khatuya, Haripada
Priority Number: US20090281307A1
IPC Current: C07D040312 | A61K0031454 | A61K00314545 | A61K0031496 | C07D023518 | C07D040104 | C07D040112 | C07D040114
US Class: 540575 | 544370 | 546194 | 546199
Title: Benzoimidazole compounds
Usefulness: Benzoimidazole compounds
Summary: For treating allergy, asthma, chronic obstructive pulmonary disease, atherosclerosis, rheumatoid arthritis, multiple sclerosis, inflammatory bowel disease, ulcerative colitis, psoriasis, scleroderma, autoimmune thyroid disease, immune-mediated diabetes mellitus, lupus, myasthenia gravis, autoimmune uveitis, autoimmune hemolytic anemia, temporal arteritis, Behcet's disease, autoimmune hepatitis, and autoimmune oophoritis.
Novelty: New benzoimidazole compound useful for treating or preventing disorders and conditions mediated by histamine receptor e.g. inflammation, atherosclerosis, rheumatoid arthritis

Industry

Chemical/Material

Sub Category

Chemical/Material Application

Application No.

9304234

Others

Related Materials

• Plasmonic Dark Field Microscopy, Appl. Phys. Lett. 96, 113107 (2010); see http://circuit.ucsd.edu/~zhaowei/Journals/APL_Hu.pdf

Tech ID/UC Case

21345/2009-289-0

Related Cases

2009-289-0

*Abstract

Dark field (DF) microscopy is widely used to view objects that have low contrast in bright-field microscopy, e.g., live and unstained biological samples. In conventional DF microscopy, the central part of the illumination light that ordinarily passes through and around the sample is blocked by a light stop, allowing only oblique rays to strike the sample. While conventional DF microscopy can achieve high contrast imaging, its resolution may also be improved by using a high numerical aperture (NA) configuration of the condenser/objective pair. However, the NA of the objective cannot be larger than that of the condenser to avoid having the oblique illuminating rays enter the objective. Also high NA condensers are very sensitive to alignment and must be accurately positioned and aligned to the very sharp cone of illumination, making them difficult to use. In addition, the illumination light in such a high NA arrangement must be very bright, which is wasteful of energy. Thus conventional DF microscopy is instrumentally bulky, complex, and costly.

*IP Issue Date

Apr 5, 2016

*Principal Investigator

Name: Zhaowei Liu

Department:

Name: Feifei Wei

Department:

Country/Region

USA