Metal-Enhanced Fluorescence Surface Acoustic Wave Device
- Technology Benefits
- Capable of detecting antigen at lower concentrationCost efficient and quick.
- Technology Application
- protein detection, antigen detection
- Detailed Technology Description
- Metal-enhanced fluorescence (MEF) is a good way to lower the detection limit. The problem is, MEF not only increases the intensity of the specifically-bound proteins, but also the intensity of the nonspecifically bound proteins (NSBPs) which could autofluoresce at the same wavelength. To remove these non-specifically bound proteins but still detect biomarkers in ng/mL concentrations, a SAW device can be used to remove the NSBPs. As an added bonus, the SAW device can double in usefulness by also causing micro-mixing, which improves attachment times. Additionally, for ng/mL biomarker concentrations, only one experiment is needed to discover concentration and location of antigens. Previously, to do both, an ELISA assay and an immunofluorescence assay would both need to be performed. This method allows for concentration and location to be discovered more quickly and more costeffectively.
- *Abstract
-
Researchers at the University of South Florida have developed a metal-enhancedfluorescence surface acoustic wave (MEF-SAW) device for the detection of proteins and antigens in real matrices such as blood and urine.
- Country/Region
- USA
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