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Endoribonucleases For Rna Detection And Analysis

Technology Application

Purifying a target RNA in a mixed population of nucleic acids Detection of specific sequences in a target polyribonucleotide Regulating expression of a target RNA in a eukaryotic cell

Detailed Technology Description

None

Application No.

9688971

Others

Publication

RNA-protein analysis using a conditional CRISPR nuclease 

Related Materials

Mechanism of substrate selection by a highly specific CRISPR endoribonuclease

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• Compositions and Methods of Use for Variant Csy4 Endoribonucleases

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• Structure-Guided Methods Of Cas9-Mediated Genome Engineering
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• Efficient Site-Specific Integration Of New Genetic Information Into Human Cells
• Cas9 Variants With Altered DNA Cleaving Activity
• Split-Cas9 For Regulatable Genome Engineering
• Single-Stranded Nucleic Acid Detection And Imaging System Using Cas9
• Methods For High Signal-To-Noise Imaging Of Chromosomal Loci In Cells Using Fluorescent Cas9
• Identification Of Sites For Internal Insertions Into Cas9
• Cas13a/C2c2 - A Dual Function Programmable RNA Endoribonuclease
• Small Molecule Assisted Cell Penetrating Cas9 RNP Delivery
• THERMOSTABLE RNA-GUIDED ENDONUCLEASES AND METHODS OF USE THEREOF (GeoCas9)
• Cas12c/C2C3 Compositions and Methods of Use
• A Dual-RNA Guided CasZ Gene Editing Technology

Tech ID/UC Case

29125/2012-124-0

Related Cases

2012-124-0

*Abstract

Bacteria and archaea possess adaptive immune systems that rely on small RNAs for defense against invasive genetic elements. CRISPR (clustered regularly interspaced short palindromic repeats) genomic loci are transcribed as long precursor RNAs, which must be enzymatically cleaved to generate mature CRISPR-derived RNAs (crRNAs) that serve as guides for foreign nucleic acid targeting and degradation. This processing occurs within the repetitive sequence and is catalyzed by a dedicated CRISPR-associated (Cas) family member in many CRISPR systems.  Endoribonucleases that process CRISPR transcripts are bacterial or archaeal enzymes capable of catalyzing sequence- and structure- specific cleavage of a single- stranded RNA. These enzymes cleave a specific phosphodiester bond within a specific RNA sequence. 

UC Berkeley researchers discovered variant Cas endoribonucleases, nucleic acids encoding the variant Cas endoribonucleases, and host cells genetically modified with the nucleic acids that can be used, potentially in conjunction with Cas9, to detect a specific sequence in a target polyribonucleotide and of regulating production of a target RNA in a eukaryotic cell.  For example, it was found that the variant Cas endoribonuclease has an amino acid substitution at a histidine residue such that is is enzymatically inactive in the absence of imidazole and is activatable in the presence of imidazole.

 

*IP Issue Date

Jun 27, 2017

*Principal Investigator

Name: Jennifer Doudna

Department:

Name: Martin Jinek

Department:

Name: Samuel Sternberg

Department:

Country/Region

USA