Mouse anti-tomato spotted wilt virus (TSWV) nucleocapsid protein antibodies (2 lines)
- Detailed Technology Description
- Applications Tomato spotted wilt tospovirus (TSWV) is the type member ofthe plant-infecting viruses of the genus Tospovirus in the family Bunyaviridae.TSWV is transmitted by thrips (Thysanoptera: Thripidae) to over 650 differentplant species in temperate and tropical regions. Once the plants infected withthe virus, they result in spotting, wilting, reduced vegetative output anddeath of the plant. Some of the crops known to be affected by the virusesinclude, peanuts, watermelons, tomatoes, zucchinis and others. Suitable forDAS-ELISA, PAS-ELISA and DIBA applications.
- *Abstract
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ReagentsDescription
Clone Name
Antigen
Accession #
Isotype
Reactivity/Recognition
1-7B4
Nucleocapsid protein of TSWV
5IP1 (RSCB PDB)
Q88910 (UniProt)
IgG2b
TSWV, possibly other Tospoviruses or Bunyaviridae. Very small cross-reactivity with WSMV and PVY
NH3-E5
Nucleocapsid protein of TSWV from multiple hosts
IgG2b
Specific for TSWV N protein.
SpeciesImmunized: Mouse
PurificationMethod: Protein G column
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl,0.05% (w/v) Sodium Azide
Tested Applications: 1-7B4: DAS-ELISA (1:100-10,000), PAS-ELISA (1:100-10,000), DIBA
NH3-E5: ELISA, DIBA, WB, DAS-ELISA,PAS-ELISA (typical dilutions are 1:1000-1:2000)
Antibodystorage temperature: -20oC
References
Bandla, MD,Daphne, MW; Chenault, K.D.; Ullman, DE; German, TL; Sherwood, JL: Use ofMonoclonal Antibody to the Nonstructural Protein Encoded by the Small RNA ofTomato Spotted Wilt Tospovirus to Identify Viruliferous Thirps: Phytopathology,84(12), 1427-1431 (1994).
Naidu, RA;Sherwood, JL; Deom, CM. Characterization of a vector-non-transmissible isolateof Tomato spotted wilt virus. Plant Pathol,57, 190-200 (2008).
Sherwood, JL,Sanborn, MR, Keyser, GC; Myers, LD. Use of Monoclonal Antibodies in Detectionof Tomato Spotted Wilt Virus, Phytopathology,79(1), 61-64 (1989).
Richmond, KE,Chenault, K; Sherwood, JL; Geramn, TL: Characterization of the Nucleic AcidBinding Properties of Tomato Spotted Wilt Virus Nucleocapsid Protein, Virology 248,6-11 (1998).
Adkins,S, Choi, T-J,Israel, BA, Bandla, MD, Richmond, KE, Schultz, KT, Sherwood, JL,German, TL. Baculovirus Expression and Processing of Tomato Spotted WiltTopovirus Glycoproteins. Phytopathology86(8), 849-855 (1996).
- Country/Region
- USA
