Monoclonal Antibodies against dorsal and sub-ventral structures in Meloidogyne spp. (Melodogynidae, "Root-knot nematode"), 13 lines
- Detailed Technology Description
- ApplicationsMembers of the genus Meloidogyne (Chitwood;over 90 species), the root-knot nematodes (roundworm), are an agronomicallyimportant group of pathogens that establish a complex parasitic relationshipwith a wide range of host plants. Currently, well over 2000 plants are susceptible to root-knot nematodeinfections, leading to over 5% of global crop loss. Substances produced in theesophageal glands of Chitwood are secreted through the stylets of feedingroot-knot nematodes, modifying several root protophloem cells into elaboratefeeding sites called giant-cells. Nutrients from the giant-cells are requiredfor the growth and pronounced morphological changes that occur during nematodedevelopment from a motile, vermiform juvenile to an enlarged, rounded,sedentary adult female. These changes include a reduction in size of the twosubventral esophageal gland cells and an increase in size of the single dorsalesophageal gland cell as the nematode develops to an adult female. The development of monoclonal antibodies mayhelp elucidate the function of several nematodal structures in organismaldevelopment and plant parasitism byfacilitating isolation of proteins from complex mixtures and aid in the developmentof counter-measures. All nematodes passthrough an embryonic stage, four juvenile stages (J1–J4) and an adult stage. Givencertain similarities of Mab binding across multiple species of the Nematodaphylum, these antibodies may also besuitable for the study of development of, and parasitism by, other members ofthe phylum. Applicable inimmunostaining, ELISA
- *Abstract
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ReagentsDescription
Clone Name
Isotype
Antigen name
Reactivity
6D4E8
IgM
Dorsal/esophageal granules of Meloidogyne spp.
M. incognita (J2 and adult stages)
M. javanica J2 stage
M arenaria J2 stage
3H11
IgG
Subventral granules of Meloidogyne spp
Only J2 stages of
M. incognita, M. javanica, and M arenaria. Possibly M hapla.
No binding to adults
1E12
IgG
Dorsal gland lobe, extension and ampulla (DG)
Meloidogyne spp., female
Negative binding for any stage of M. hapla, H. glycines and C. elegans
1D9
IgG
Dorsal granules (DG)
Meloidogyne spp., female
6F11
IgG
DG
Meloidogyne spp., female
8D12
IgM
Somatic muscles of H. glycines (J2 stage)
H. glycines (J2, adult female).
C. elegans
3F4
IgM
Secretory granules within the subventral gland extensions and the ampulla in the metacorpus. Subventral granules (SvG) of J2;
SvG, amphids and esophageal lumen of females
M incognita (J2 stage), M. haplas, M. javanica, and M arenaria
12H7
IgG
Hypodermal chords (J2);
Dorsal gland lobe , extension and ampulla of adult females (DG)
M. incognita, M arenaria, M. javanica.
M.hapla (J2 only- hypodermal chords; DG-females only)
7E1
IgM
Esophago-intestinal cells immediately posterior to metacorpus in M. incognita (female and J2)
Meloidogyne spp., female and J2
2G9
IgM
muscle
Meloidogyne spp., H. glycines. (both female and J2)
C elegans
11D11
IgG
Hypodermal chords (J2);
Amphids (females)
J2 of Meloidogyne spp. and H. glycines. C. elegans. Females of Meloidogyne spp.
11D4
IgM
Excretory canals
Females of Meloidogyne spp.; No biding to J2
11F3
IgM
Muscle ( J2 only);
Metacorpal pump chamber, esophageal lumen, stylet knobs
Muscle (J2) and metacorpal pump (female): Meloidogyne spp. and H. glycines
References
Davis EL, Aron,LM, Pratt, LH, Hussey, RS: Novel immunization Procedures used to DevelopMonoclonal Antibodies that Bind to Specific Structures in Meloidogyne spp.(1992). Phytopathology,82(10), 1244-1250.
Hussey, R.S.:Monoclonal Antibodies to Secretory Granules in Esophageal Glands in MeloidogyneSpecies. (1989) J. Nematology,21(3), 392-398
Ray, C, AbbottAG, Hussey, RS: Trans-splicing of a Meloidogyne incognita mRNA encoding aputative esophageal gland protein. (1994). Mol. Biochem. Parasitology , 68, 93-101
Hussey, R. S.& Grundler, F. M. W. 1998 Nematodeparasitism of plants. In: The Physiology and Biochemistry of free-living andplant-parasitic nematodes. Perry, R. N. & Wright, D. J. (Eds), CABI Publishing,UK. pp 213 – 243
Hussey, RS,Paguio, OR, Seabury, F: (1990) Localization and purification of a secretoryprotein from the esophageal glands of Meloidogyneincognita with a monoclonal antibody. Phytopathology,80, 709-714
- Country/Region
- USA
