Anti-Bordotella avium 41-kDa Surface Protein Antibodies (3 lines)
- Detailed Technology Description
- Applications Bordotella avium, a Gram-negative, aerobic and motilebacterium, is the causative agent of turkey coryza, an economically-impactingdisease that can also infect other birds. The bacterium has been isolated fromindividuals with cystic fibrosis, although the contribution of the organism tothe disease is still unclear. B. aviumis consistently resistant to penicillin and cefuroxime, but susceptible, invarious degrees, to mezlocillin, piperacillin, gentamicin, amikacin, andcefoperazone. B. avium shares severalvirulence factors with human pathogens such as B. pertussis, B.parapertussis, and B. bronchisepticaand is, in fact, phylogenetically (16S rRNA) closely related to theseorganisms. A 41 kDa surface protein of B.avium is strongly associated with the organism’s virulence and ability toattach to tracheal mucosa. Monclonal antibodies designated 9F8, 1G12 and 14G8immunoprecipitate the 41kDa protein and also inhibited HA of guine pigeruthrocytes by B. avium (HI titer of1024 (9F8 and 1G12) and 512 for 14G8). These antibodies have the potential tobe used in the development of therapeutic and/or diagnostic tools. Validated for ELISA, WB and IF applications.
- *Abstract
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Reagent Description
Antigen:
Bordotella avium 41-kDa Surface Protein
Clone Name:
1G12.B4 (IgG1k); 14G8 (IgG3k); 9F8 (IgG1k)
Reactivity:
B. avium possibly other Bordetella
Immunogen:
Protein
Species Immunized:
Mouse
Buffer:
Cell Culture Supernatant
Tested Applications:
ELISA, WB and IF
References
Moore, KM, Jackwood MW:(1994) Production of Monoclonal Antibodies to the Bordotella avium 41-Kilodalton Surface Protein and Characterizationof the Hemagglutinin. AvianDiseases, 38(2, Apr.- Jun.), 218-224
Domingo, DT, Jackwood MW,Brown, TP: (1992) Filamentous Forms of Bordotella avium: Culture Conditions andPathogenicity. AvianDiseases 36, 707-713
Moore, KM, Jackwood, MW,Brown, TP, Dreesen, DW: (1994) Bordoteliaavium Hemagglutination and Motility Mutants: Isolation, Characterizationand Pathogenicity. AvianDiseases 38, 50-58
Jackwood, MW, Hilt, DA,Dunn, PA: (1991) Observations on Colonial Phenotypic Variation in Bordotella avium. AvianDiseases, 35, 496-504
Jackwood, MW, McCarter, SM,Brwon, TP: Bordetella avium: AnOpportunistic Pathogen in Leghorn Chickens. (1992). AvianDiseases, Vol. 36, No. 3 (Jul. - Sep., 1992), pp. 707-713
HarringtonA.T, Castellanos, JA, Ziedalski, TM, Claridge III, JE, Crockson, BT: (2009)Isolation of Bordotella avium andNovel Bordotella Strain from Patients with Respiratory Disease. Emerg.Infect. Diseases 15(1), 72-74
- Country/Region
- USA
