Anti- Eukaryotic Actin Related Proteins (ARP) Monoclonal Antibodies (2 lines)
- Detailed Technology Description
- Lines 12A2.A3.C2 and 12B2.G4.E2.C4 recognize Actin-RelatedProtein 6 (ARP6) while line 13F10.A4.G6 binds to the C-terminal amino acids ofARP8 and line 12F5.D4.C3 recognizes the N-terminal amino acids of ARP8. Alllines have been validated for use in WB, ELISA, IF,IB and Immunocytochemistry.Actin-related proteins (ARPs) are found in the nuclei of alleukaryotic cells, but their functions are generally understood only in thecontext of their presence in various yeast and animal chromatin-modifyingcomplexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actinand Arabidopsis ACT2 in the regions of alignment, respectively. Because it isnot closely related to yeast or human ARP8 and shows similar weak homology toyeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphanARP. Arabidopsis thaliana ARP6 is a clear homolog of other eukaryotic ARP6s,including Saccharomyces cerevisiaeARP6, which was identified as a component of the SWR1 chromatin remodelingcomplex. Arabidopsis ARP6 is localized to the nucleus during interphase butdispersed away from the chromosomes during cell division. ARP6 expression wasobserved in all vegetative tissues as well as in a subset of reproductivetissues. Null mutations in ARP6 caused numerous defects, including altereddevelopment of the leaf, inflorescence, and flower as well as reduced femalefertility and early flowering in both long- and short-day photoperiods.
- *Abstract
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ReagentsDescription
Clone Name
Antigen
Accession #
MW (kDa)
Isotype
Immunogen (Epitope)
Reactivity/Recognition
12A2.A3.C2
ARP6
Q8LGE3
47
IgG1
ARP6
ARP6
12B2.G4.E2.C4
ARP6
Q8LGE3
47
IgG1
ARP6
ARP6
13F10.A4.G6
ARP8 including rec-forms.
Q9FKT0
52
IgG1
ARP8
(N-Terminal: aa 2-ILKKVWG SVWNRSNSGKDLVNHQRA-26)
A. thaliana, Brassica and other eukaryotes for which ARP8 is closely related to thaliana's
12F5.D4.C3
ARP8 including rec-forms.
Q9FKT0
52
IgG1
ARP8
(C-Terminal: aa 447-SNLSIFPGPWCITRKQFRRKSRLMW-471)
A. thaliana, Brassica and other eukaryotes for which ARP8 is closely related to thaliana's
SpeciesImmunized: Mouse
PurificationMethod: Protein G column
Buffer: 0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl,0.05% (w/v) Sodium Azide
TestedApplications: WB, ELISA, IF, IB andImmunocytochemistry
Antibodystorage temperature: -80oC
References
Kandasamy MK,McKinney EC, Meagher RB. ACTIN-RELATED PROTEIN8 encodes an F-box proteinlocalized to the nucleolus in Arabidopsis. Plant CellPhysiol. 2008 May;49(5):858-63.
Li Y, KandasamyMK, Meagher RB. Rapid isolation of monoclonal antibodies. Monitoring enzymes inthe phytochelatin synthesis pathway. Plant Physiol.2001 Nov;127(3):711-9.
Kandasamy MK,McKinney EC, Meagher RB. Differential sublocalization of actin variants withinthe nucleus. Cytoskeleton(Hoboken). 2010 Nov;67(11):729-43.
Meagher RB,Kandasamy MK, Smith AP, McKinney EC. Nuclear actin-related proteins at the coreof epigenetic control. Plant SignalBehav. 2010 May;5(5):518-22.
Richard B.Meagher, Roger B. Deal, Muthugapatti K. Kandasamy and Elizabeth C. McKinney:Nuclear Actin-Related Proteins as Epigenetic Regulators of Development. Plant PhysiologyDecember 2005 vol. 139 no. 4 , 1576-1585
Kristó I, BajuszI, Bajusz C, Borkúti P, Vilmos P. Actin, actin-binding proteins, andactin-related proteins in the nucleus. Histochem CellBiol. 2016 Apr;145(4):373-88
- Country/Region
- USA
