High--Throughput Flow Cytometry on Adherent Cells
- Detailed Technology Description
- This method offers a simple alternative to cumbersome protocols currently in use for preparing adherent cell lines for medium to highthroughput screening by flow cytometry. Routinely used protocols on adherent cells include various wash and centrifugation steps, which are not only time consuming and low-throughput but also result in cell number loss (in our experience, up to 85% in four washes). Our method offers lifting of adherent cells without removing media, eliminates wash steps, and prevents cell loss; thus allowing high-throughput flow cytometry on adherent cells in 96 and 384-well plates.
- *Abstract
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Flow cytometry is a widely used technology having applications in various areas of research. One of the key requirements of using this technology lies in the use of nonadherent/floating cells that are passed through lasers for obtaining relevant data. Many of the routinely used cell culture models are adherent in nature and need several timeconsuming steps before they can be used for flow cytometry. This includes removal of growth medium, adding cell lifting solution, cell washing using multiple centrifugation steps, resuspension of cells in appropriate media, staining, and analysis using flow cytometry. Our method lifts cells within the same culture plate without undergoing any of the abovementioned steps, preventing cell loss and providing fast, hasslefree sample preparation for flow cytometry.
- Country/Region
- USA
