Non-Invasive Real-Time Immune Response Monitoring
- Technology Application
- Tumor profilingTCR clonotyping in rheumatoid arthritis,certain tumors, ankylosing spondylitis, CMV and EBV infections, respectively
- Detailed Technology Description
- A novel method for T-cell receptors and immunoglobulin clonotypingusing a new hybrid capture method coupled to DNA sequencing
- *Abstract
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Through a new hybrid method, the ability toclonotype T-cell receptor (TCR) gene segments and immunoglobulins (Ig) has beenenabled. Current technology relies onhigh-throughput PCR amplification from blood cells carrying target DNA. New clonotyping of TCRs described hereidentify gene targets directly from a library, which also allows for tumorcharacterization in parallel. Furthermore, the new technique is applicable to detection from blood plasma. Thus eliminating the need for extensive PCRamplification and reducing the cost of analysis by 10 – 20 X below currentmethods, leading to expanded capabilities to perform high-throughput analysisat reduced cost. Cell-free applicationsoffer sensitive, less invasive diagnostics with the ability to detect theorigin of DNA from diseased cells from a variety of tissues. Cancer diagnostics and immunotherapy is arapidly expanding sector and would benefit from this novel methodology thatperforms clonotyping and tumor characterization in parallel.
Anoverview of immunotherapy strategies and the important role of the TCR. The invention provides a novel approach tobarcoding the TCR for better therapeutic design Adapted from (Humphries, 2013).
- *Principal Investigator
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Dr. Trevor Pugh, Princess Margaret CancerCentre, University Health Network
- *Publications
- Humphries, C. (2013) Adoptive cell therapy: Honing that killer instinct. Nature Dec 19; 504(7480):S13–S15
- Country/Region
- USA
