Anti-Ciona Brachyury Polyclonal Antibody
- Detailed Technology Description
- This invention provides a rabbit polyclonal antibody against Ciona Brachyury, a transcriptional factor playing an important role in mesoderm formation in animals.
- Others
-
- Functional Brachyury binding sites establish a temporal read-out of gene expression in the Ciona notochord. PLoS Biol., 2013:e1001697.
- Optimization of a method for chromatin immunoprecipitation assays in the marine invertebrate chordate Ciona. Mar Biotechnol , 2013,15, 520-525.
- *Abstract
-
The transcription factor Brachyury plays a key role in mesoderm formation and is required for the formation of notochord from axial mesoderm in animals. Recently, Brachyury has also been shown as a causative agent of chordomas, human tumors of presumed notochordal origin.
Cornell inventors have generated a rabbit polyclonal antibody against the full-length protein of Ciona Brachyury. The inventors have successfully used this antibody in immunohistochemistry and chromatin IP (ChIP) in their own research. This antibody works very specifically in Ciona and might potentially cross react with related proteins from other organisms.
Figure 1. Fluorescence (A-D) and Nomarski (E) microphotographs of a late tailbud Ciona embryo electroporated at the 1-cell stage with the Ci-Bra>eGFP plasmid (Corbo et al., 1997) and immunostained with a Ci-Bra-specific antibody. (A) Red channel, showing all nuclei of the 40 notochord cells stained by the antibody. (B) Green channel, showing eGFP expression in only a subset of the 40 notochord cells, due to mosaic incorporation of the Ci-Bra>eGFP plasmid. (C) Blue channel, showing nuclear DAPI staining of epidermis and notochord cells. (D) Merge of the photographs in (A-C). (E) Bright-field view of the embryo.
Potential Application
A research reagent
- *Licensing
- Vibhu Sachdev(212) 746-6187sachdev@cornell.edu
- Country/Region
- USA
