Monoclonal Antibodies to Tctex-1 (M17)
- Others
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- Sachdev, P., Menon, S., Kastner, D.B., Chuang, J.Z., Yeh, T.Y., Conde, C., Caceres, A., Sung, C.H., and Sakmar, T.P. (2007). G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth. Embo J 26, 2621-2632.
- Dedesma, C., Chuang, J.Z., Alfinito, P.D., and Sung, C.H. (2006). Dynein light chain Tctex-1 identifies neural progenitors in adult brain. J Comp Neurol 496, 773-786.
- Gauthier-Fisher, A., Lin, D.C., Greeve, M., Kaplan, D.R., Rottapel, R., and Miller, F.D. (2009). Lfc and Tctex-1 regulate the genesis of neurons from cortical precursor cells. Nat Neurosci 12, 735-744.
- Chuang, J.Z., Yeh, T.Y., Bollati, F., Conde, C., Canavosio, F., Caceres, A., and Sung, C.H. (2005). The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth. Developmental cell 9, 75-86.
- Vlach, J., Lipov, J., Rumlova, M., Veverka, V., Lang, J., Srb, P., Knejzlik, Z., Pichova, I., Hunter, E., Hrabal, R., et al. (2008). D-retrovirus morphogenetic switch driven by the targeting signal accessibility to Tctex-1 of dynein. Proc Natl Acad Sci U S A 105, 10565-10570.
- Lai, M., Wang, F., Rohan, J.G., Maeno-Hikichi, Y., Chen, Y., Zhou, Y., Gao, G., Sather, W.A., and Zhang, J.F. (2005). A tctex1-Ca2+ channel complex for selective surface expression of Ca2+ channels in neurons. Nat Neurosci 8, 435-442.
- King, S.M., Dillman, J.F., 3rd, Benashski, S.E., Lye, R.J., Patel-King, R.S., and Pfister, K.K. (1996). The mouse t-complex-encoded protein Tctex-1 is a light chain of brain cytoplasmic dynein. J Biol Chem 271, 32281-32287.
- Machado, R.D., Rudarakanchana, N., Atkinson, C., Flanagan, J.A., Harrison, R., Morrell, N.W., and Trembath, R.C. (2003). Functional interaction between BMPR-II and Tctex-1, a light chain of Dynein, is isoform-specific and disrupted by mutations underlying primary pulmonary hypertension. Hum Mol Genet 12, 3277-3286.
- *Abstract
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Mouse hybridoma (clone M17) for production of a monoclonal antibody (mAb) against full-length mouse tctex-1 (1). Tctex-1 is the light subunit of dynein, involved in cargo-binding in neurons. Tctex-1 was recently shown to be a marker of neural progenitor cells (2). The mAb recognizes an N-terminal epitope on tctex-1. Mouse tctex-1 shares 93% amino acid sequence identity with its human homolog. The mAb cross-reacts with tctex-1 of rat, and human origin.
Anti-tctex-1 antibodies have been used as neuroscience reagents to study neurogenesis (3), and cytoskeletal remodeling (4) and dynamics (5,6). However, due to the wide organ expression of Tctex-1 (EST profile is here) (7) anti-Tctex-1 mAbs are also useful reagents outside of the field of neuroscience (8).
Anti-tctex-1 mAbs applications: Western blot, immunoprecipitation, immunohistochemistry, immunofluorescence.
Modified Figure excerpt from (1). Immunofluorescent staining of a developmental stage 3 cultured primary mouse hippocampal neuron with anti-tctex-1 mAb and an Alexa 594-conjugated goat anti-mouse secondary antibody. Arrows point at tctex-1 staining within the cell body and growth cone.
If you are with a reagent company and would be interested in adding this to your product line, please contact Dan-Oscar Antson, as per the contact information below.
- *Licensing
- Dan-Oscar Antsonda429@cornell.edu212-746-1297
- Country/Region
- USA
