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A Genetic Selection for Protein Folding and Solubility in the Bacterial Periplasm

Detailed Technology Description

The invention provides a technique for analyzing protein folding and solubility in a compartment of the cell called the periplasm

Others

Mansell TJ, FIsher AC and DeLisa MP (2007) A protein folding and solubility reporter for the bacterial periplasm (in preparation).

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*Abstract

The invention provides a technique for analyzing protein folding and solubility in a compartment of the cell called the periplasm. The technology correlates protein folding with the activity of a reporter, such as antibiotic resistance. This method can be used to identify potential drug candidates for diseases such as Alzheimers disease, Parkinsons disease, and Huntingtons disease that are associated with protein misfolding.

POTENTIAL APPLICATIONS:

• Protein drug screening and screening of directed-evolution libraries as well as traditional libraries of periplasmic proteins
• Evaluation of the optimal folding environment for recombinant proteins
• Observation of the different folding environments of the cytoplasm and periplasm
• Generation of mutations to improve the folding and solubility of valuable proteins.

ADVANTAGES:

• The invention is unique in its ability to characterize folding and solubility of proteins in the periplasm. Current technologies focus on protein folding in the cytoplasm, so there currently exists no method to monitor protein folding in the periplasm.
• Many proteins in the periplasm are commercially important.
• The invention can be a powerful tool for drug discovery, research, and protein production.

*Licensing

Alice (Xiang) Li, Senior Technology Commercialization and Liaison Officerxl11@cornell.edu607-254-4487

Country/Region

USA