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Time-Resolved Fluorescence Imaging Without Lifetime Fitting

Technology Benefits

Provides rapid determination of the relative lifetime within an imageSimpler and more reliable

Technology Application

Cancer detection in a broad range of imaging proceduresCosmetic application for determination of collagen and elastin ratioIdentification of unknown substances in medical forensics

Detailed Technology Description

Researchers at UCLA have developed a method to obtain time-resolved fluorescence imaging (TRFI) without the need of lifetime fitting. The method first utilizes the lifetime differences of fluorophores to normalize images sampled at multiple fluorescence decay time points. The method then applies division and intensification on images sampled when all the fluorophores are still fluorescing.

Others

State Of Development

The capability of both methods has been tested by using Fluorescein and Rhodamine-B as samples. Both methods show the ability to distinguish fluorophores from each other. In addition, the methods can avoid the effect of non-uniformity of the fluorescence intensity.

Background

Time-resolved fluorescence imaging is an effective tool to study complex biological samples. In medicine, the distribution in space of any fluorescent marker can be exploited for diagnostic purposes. Images using this technique are acquired by obtaining the lifetime pattern of the sample. This is complicated due to the fact that lifetime-extraction to obtain fluorescence images may not be reliable.Fluorescence decay fitting and lifetime estimation methods have resolution limits, especially when it comes to multiple fluorophores. A simple and accurate method to obtain fluorescence image without lifetime-extraction is a desirable option for time-resolved fluorescence imaging.

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Tech ID/UC Case

27548/2010-142-0

Related Cases

2010-142-0

*Abstract

UCLA investigators have developed a novel method to obtain time-resolved fluorescence imaging (TRFI) without the need to extract a fluorescence lifetime. Compared to conventional TRFI, this novel method is reliable, simple, time-saving and can dramatically improve biomedical applications of TRFI.

*Principal Investigator

Name: Warren Grundfest

Department:

Name: Pei-Chi Jiang

Department:

Name: Asael Papour

Department:

Name: Oscar Stafsudd

Department:

Name: Zachary Taylor

Department:

Name: Maie St John

Department:

Country/Region

USA