Induction of Corneal Endothelial Cells
Unlike previously reported induction procedures, the present methods utilize a novel small molecule-based approach to derive human CECs along with three major retinal cell types, RPE, photoreceptor and RGC, from PSCs in a directional fate restriction fashion. Moreover, using EFSC-derived NCSCs as a starting cell source cell, CECs can be quickly and directly induced and expanded in culture.
A therapeutic composition and method for treating a patient in need of ocular therapy.A research tool comprising a feeder-free and serum-free culture of corneal endothelial cells (CECs). A method of identifying a pharmaceutically active agent, comprising combining a pharmaceutical candidate with a feeder-free and serum-free culture of corneal endothelial cells (CECs) and detecting a desired pharmaceutical activity, thereby identifying a pharmaceutically active agent.
Researchers at UC San Diego have developed a small molecule based methodology to derive human CESs from PSCs via ocular lineage specification. A three-step strategy was employed; [1] eye field stem cells (EFSCs) were derived from PSCs, (2) EFSCs were directed to ocular neural crest stem cells (NCSC) fate, and (3) differentiated ocular NCSCs to human corneal endothelial cells (CECs) resulting in a homogeneous and expandable monolayer of CECs in culture.
State Of Development The current methodology provides a research tool for generating feeder and serum-free culture of CDCs. The majority of CECs expressed ZO-1, N-cadherin and Na+/K+ATPase, all characteristic markers of CECs. Intellectual Property Info Patent-pending. Worldwide rights are available. Related Materials Tech ID/UC Case 27338/2016-284-0 Related Cases 2016-284-0
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