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Kit for Identification of Traces of High Explosives Using Thin Layer Chromatography

Detailed Technology Description

UC San Diego researchers have developed sensors, sensor fabrication processes, and sensing methods that enable detecting traces of high explosives by fluorescence quenching in combination with chromatographic separation. The invention includes use of fluorescent, silicon based copolymers, covalently linked to silica thin-layer chromatographic plates or nanoparticle supports. A thin layer of the polymer allows for a more efficient interaction with an analyte, thus yielding enhanced detection sensitivity. The attachment of the sensing polymers onto a chromatographic support allows for the separation of a mixture, as well as the identification of multiple explosives through the use of multiple sensing polymers. Coating silica nanoparticles with the sensing polymer provides a simple means of detecting high explosives in aqueous media with a low detection limit. The invention can be implemented as inexpensive field-ready kits for the identification of trace explosives.

Supplementary Information

Inventor: Zhang, Linqi | Lewin, Sharon R. | Kostrikis, Leondios | Ho, David D.
Priority Number: US6235504B1
IPC Current: C12Q000168 | C12Q000170
US Class: 4350912 | 4350911 | 5360231 | 5360243 | 53602431 | 53602432 | 53602433 | 53602532
Assignee Applicant: The Rockefeller University,New York
Title: Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
Usefulness: Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein
Summary: The method is useful for determining the number of copies per cell of at least one preselected polynucleotide target sequence in a nucleic acid sample of cellular origin. The method is specifically useful in monitoring T cell receptor circle sequences, where the method provides an indication of the recovery of the immune system with various therapies; in monitoring thymic function with age; and in quantifying viral, microbial or cellular sequences. The genomic equivalent marker may be used to determine the number of copies of a marker of thymocyte proliferation, to identify other genetic sequences for use as genomic equivalent markers, and to facilitate denotation of target cellular nucleic acid levels as abundance per cell;
Novelty: Determining the number of copies per cell of a preselected polynucleotide target sequence, for monitoring immune system recovery, comprises quantifying at least one target sequence and at least one genomic equivalent marker sequence

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

9134239

Others

State Of Development

This technology has a working prototype.

Intellectual Property Info

The invention has a patent pending and is available for licensing.

Related Materials

Hollow Silica Nanospheres Containing a Silafluorene-Fluorene Conjugated Polymer for Aqueous TNT and RDX Detection, Chem. Commun., 2010, 46, 6804-6806.
Luminescent Organosilicon Polymers and Sol-Gel Synthesis of Nano-Structured Silica, Martinez, H. Paul, Ph.D., Dissertation, University of California, San Diego, 2011.

Tech ID/UC Case

21905/2011-061-0

Related Cases

2011-061-0

*Abstract

Explosives detection is critically important in many field settings (e.g., military facilities, minefields, crime scenes, and remediation sites) and has become a necessity for the safety of the general public (e.g., at airports and mass transit areas). As such, there remains a demand for inexpensive and reliable explosive sensors for identifying specific explosives. High explosives are considered to be organic and oxidizing, a relatively rare combination that makes them tractable for molecular recognition. Fluorescent polymers have thus had favorable success in their use as high explosive sensors.

*IP Issue Date

Sep 15, 2015

*Principal Investigator

Name: H Martinez

Department:

Name: William Trogler

Department:

Country/Region

USA