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T7 Transcription Enhancing Sequence (TEnBOX), Which Overexpresses Gene Products

Technology Application

Production of recombinant protein or gene products in bacteria or other cells is dramatically increased when the TEnBOX is incorporated into the plasmid vector. The efficiency of the protein expression from the TEnBOX is much higher than reported with the best commercial vectors (pET-21 and pTriEX-3) or with the best translation enhancing elements or downstream boxes. Another version is regulated by IPTG and is useful for expressing proteins that are toxic to E. coli. It is not leaky and its expression is tightly regulated by IPTG. High-yield production of recombinant proteins, such as growth hormones, peptides, enzymes, industrial proteins, and therapeutic proteins in host cells, can lead to obtaining high quality protein with less running cost.

Detailed Technology Description

UC San Diego researchers have discovered an enhancer function of an approximately 30 bp DNA fragment that had been reported to function as a protein transduction domain in microbial cells.

Supplementary Information

Patent Number: US8552168B2
Application Number: US2009599577A
Inventor: Briggs, Steven P. | Tachikawa, Kiyoshi
Priority Date: 11 May 2007
Priority Number: US8552168B2
Application Date: 19 Jul 2010
Publication Date: 8 Oct 2013
IPC Current: C07H002104 | C12N000100 | C12N000112 | C12N000120 | C12N001500 | C12N001574 | C12N001582 | C12P002106
US Class: 5360234 | 4350691 | 4352523 | 43525233 | 4352541 | 4352551 | 4352571 | 4353201 | 435455 | 435468 | 435471 | 800278
Assignee Applicant: The Regents of the University of California
Title: Nucleic acids for inducing expression of transcripts and proteins and methods for making and using them
Usefulness: Nucleic acids for inducing expression of transcripts and proteins and methods for making and using them
Summary: The isolated, purified, synthetic or recombinant nucleic acid and methods are useful for increased expression or increased stabilization of mRNA (transcript) and/or polypeptide; expressing an mRNA (transcript) in a cell; and for enhancing expressing an mRNA (transcript) (all claimed).
Novelty: New isolated, purified, synthetic or recombinant nucleic acid that acts as enhancer of transcription when linked to second nucleic acid sequence, useful for increased expression or stabilization of messenger RNA (mRNA) or polypeptide

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

8552168

Others

Intellectual Property Info

Pending with worldwide rights; please see U.S. application 60/917,364.

Tech ID/UC Case

20827/2007-208-0

Related Cases

2007-208-0

*Abstract

None

*IP Issue Date

Oct 8, 2013

*Principal Investigator

Name: Steven Briggs

Department:

Name: Kiyoshi Tachikawa

Department:

Country/Region

USA