Metabolic Engineering Of The Shikimate Pathway Via Synthetic Operons
Previous work has tried to change only a few pathway enzymes at a time. This more systematic approach can better optimize flux in the pathway.This invention puts all of the genes on together, simplifying the regulation and synthesis of new strains.The constructed modules ca be transferred to other strains or microorganisms or even to the chromosomes to increase stability.As a modular system, additional enzymes can be added to produce other amino acids or intermediates associated with the shikimate pathway. This process did not result in growth inhibition as commonly observed in other reported attempts to improve pathway yield.
None
Inventor: JUMINAGA, Darmawi | KEASLING, Jay, D.
Priority Number: WO2013033652A1
IPC Current: C12N000988 | C12N001563 | C12N001567
Assignee Applicant: The Regents of the University of California
Title: METABOLIC ENGINEERING OF THE SHIKIMATE PATHWAY | INGÉNIÉRIE MÉTABOLIQUE DE LA VOIE SHIKIMATE
Usefulness: METABOLIC ENGINEERING OF THE SHIKIMATE PATHWAY | INGÉNIÉRIE MÉTABOLIQUE DE LA VOIE SHIKIMATE
Summary: The host cell is useful for producing aromatic amino acid and tyrosine intermediates (all claimed), where the aromatic amino acids is useful as animal feeds, food additives, supplements, commodity chemicals and biopolymers. No biological data given.
Novelty: New host cell comprising a recombinant polynucleotide, useful for producing aromatic amino acid and tyrosine intermediates, where the aromatic amino acids is useful as animal feeds, food additives, supplements and biopolymers
Biomedical
DNA/Gene Engineering
9540652
Tech ID/UC Case 21891/2011-079-0 Related Cases 2011-079-0
USA
