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Synthetic transcriptional control elements

Technology Benefits

Easy to designOperate on entire operonsHighly amenable to development and modificationOffer a broader dynamic range for expression of the regulated nucleic acid of interest than with cis-regulators of translation aloneAllow for precise regulation of the level of expression of the nucleic acid of interest

Technology Application

Gene expression control systems for bioengineeringCustomizable regulators for gene expression

Detailed Technology Description

None

Supplementary Information

Inventor: QI, Lei S. | LIU, Chang | ARKIN, Adam P.
Priority Number: WO2013049330A1
IPC Current: C07H002102 | C12N001500
Assignee Applicant: The Regents of the University of California
Title: SYNTHETIC TRANSCRIPTIONAL CONTROL ELEMENTS AND METHODS OF GENERATING AND USING SUCH ELEMENTS | ÉLÉMENTS SYNTHÉTIQUES DE CONTRÔLE TRANSCRIPTIONNEL ET MÉTHODES DE GÉNÉRATION ET D'UTILISATION DE CES ÉLÉMENTS
Usefulness: SYNTHETIC TRANSCRIPTIONAL CONTROL ELEMENTS AND METHODS OF GENERATING AND USING SUCH ELEMENTS | ÉLÉMENTS SYNTHÉTIQUES DE CONTRÔLE TRANSCRIPTIONNEL ET MÉTHODES DE GÉNÉRATION ET D'UTILISATION DE CES ÉLÉMENTS
Summary: The nucleic acid construct, cell, method or kit is useful for converting cis-regulator of translation into synthetic TCRT. The composition and method are also useful for synthetic gene regulation. The synthetic TCRT is used to regulate the expression of a downstream nucleic acid sequence of interest.
Novelty: New nucleic acid construct useful for converting cis-regulator of translation into synthetic translation-coupled regulator of transcription, comprises synthetic translation-coupled regulator of transcription

Industry

Biomedical

Sub Category

DNA/Gene Engineering

Application No.

9593338

Others

Publication

An adaptor from translational to transcriptional control enables predictable assembly of complex regulation

Additional Technologies by these Inventors

• Regulation Of Transcription With Unnatural Amino Acid Molecules

Tech ID/UC Case

21379/2011-117-0

Related Cases

2011-117-0

*Abstract

Much of bacterium’s regulatory potential is included in 5’-untranslated regions (5’-UTRs), which control the expression of physically adjacent downstream genes.  When engineering custom regulators for 5’-UTRs, there is a tradeoff between ease of design for regulators of translation and versatility of output for regulators of transcriptional continuation.  Scientists at UC Berkeley have developed a method for converting cis-regulators of translation into synthetic translation-coupled regulators of transcription and related compositions.  The compositions and methods involve a nucleic acid construct including a synthetic translation-coupled regulator of transcription (synthetic TCRT) that includes a cis-regulator of translation coupled to an adapter.  The resulting custom regulators are easy to design, can operate on entire operons and can be composed into logics and higher-order functions.

 

*IP Issue Date

Mar 14, 2017

*Principal Investigator

Name: Adam Arkin

Department:

Name: Chang Liu

Department:

Name: Lei Qi

Department:

Country/Region

USA