Crop Improvement And Production Of Value-Added Compound Using The Rice Beta-Glucanase Genes, Gns2-Gns9
Low-cost method of production for proprietary compounds A variety of transformation methods can be used (microprojectile bombardment, Agrobacteria, electroporation, etc.) Commercial proteins can be directly expressed in roots, mature and germinated seeds, coleoptiles, and callus cells of the transgenic plant.
Genes may be used to facilitate the genetic enhancement of monocot plants with respect to plant defense and plant growth and development. Promoters fused to genes of interest expressed in transgenic plants can produce value-added compounds at reduced cost.
A researcher at the University of California Davis has isolated and characterized the nucleotide sequences and gene expression of eight Oryza sativa beta-glucanase genes which may facilitate the genetic enhancement of monocot plants including, but not limited to, the improvement of agronomic traits such as yield and tolerance to biotic and abiotic stresses. Further, the promoters may be used to produce value added compounds (e.g., proteins and small molecules of medical, industrial and nutritional importance) in transgenic monocot cells in culture, vegetative tissues and seeds. These promoters can be fused to a gene encoding a commercially important protein such as hormone, vaccine, antibody, enzyme, enzyme inhibitor, etc. The chimera can be used to create transgenic plants that can be used to produce value-added products. Because the various rice beta-glucanase genes are expressed in different tissues, at different stages of development and in response to different exogenous regulatory molecules, it may be possible to express these commercial proteins in the roots, mature and germinated seeds, coleoptiles and callus cells of the transgenic plant. The promoter for the beta-glucanase gene, Gns9, can be used to express selectable marker genes in transgenic calli and because the Gns9 gene is not expressed in the seedling, no selectable marker gene product is the vegetative tissues.
Patent Number: US6288303B1
Application Number: US1998105390A
Inventor: Rodriguez, Raymond L.
Priority Date: 25 Jun 1997
Priority Number: US6288303B1
Application Date: 25 Jun 1998
Publication Date: 11 Sep 2001
IPC Current: C07K001481 | C12N000924 | C12N001529 | C12N001556 | C12N001582
US Class: 800287 | 4350698 | 4353201 | 435468 | 5360236 | 5360241 | 800298 | 800320 | 8003202
Assignee Applicant: The Regents of the University of California
Title: Rice beta-glucanase enzymes and genes | Rice β-glucanase enzymes and genes
Usefulness: Rice beta-glucanase enzymes and genes | Rice β-glucanase enzymes and genes
Summary: USE The polynucleotides of the invention are useful to transform monocot plants. The polynucleotides and the proteins produced may also provide increased resistance to fungal infection, improved growth characteristics, and high expression levels of heterologous proteins in various tissues obtained from the plant.
Novelty: New polynucleotides which hybridise with rice beta-glucanase genes useful for transforming monocot plants for various characteristics including increased resistance to fungal infection
Biomedical
DNA/Gene Engineering
6288303
Related Materials Additional Technologies by these Inventors Tech ID/UC Case 20874/1997-276-0 Related Cases 1997-276-0
USA
