Media.Player

Method of Preparing Multivalent Single Chain Antibodies (scFv)

Technology Benefits

This method surpasses previous methods of production, such as using polyvalent proteins and antibodies, which were limited by low throughput and high cost.

Technology Application

This method provides an efficient and controllable way to produce multivalent antibodies for the advancement of scFv against cancer.

Detailed Technology Description

Single chain fragments (scFv) derived from the small binding domain of parent monoclonal antibodies (MAb) offer pharmacokinetic advantages and target tumor cells more efficiently than MAb. However, methods for producing multivalent scFv have proven to be very challenging. UC Davis researchers have recently developed a novel method to construct divalent scFv through a small linker using 1,3-dipolar cycloaddition chemistry.

Supplementary Information

Patent Number: US20090234105A1
Application Number: US2008294227A
Inventor: Gervay-Hague, Jacquelyn | Du, Wenjun | Denardo, Sally | Natarajan, Arutselvan
Priority Date: 24 Mar 2006
Priority Number: US20090234105A1
Application Date: 23 Sep 2008
Publication Date: 17 Sep 2009
IPC Current: C07K001646 | A61P003500
US Class: 5303873
Assignee Applicant: The Regents of the University of California
Title: Construction of a Multivalent SCFV Through Alkyne-Azide 1,3-Dipolar Cycloaddition
Usefulness: Construction of a Multivalent SCFV Through Alkyne-Azide 1,3-Dipolar Cycloaddition
Summary: For treating breast and prostate cancer cells expressing mucin-1 (MUC-1) antigen (claimed).
Novelty: Covalently attaching two proteins to form a conjugate protein for treating breast cancer involves contacting first protein with alkyne moieties with second protein having linking group with terminal azide moiety, to form 1,2,3-triazole

Industry

Disease Diagnostic/Treatment

Sub Category

Cancer/Tumor

Application No.

8946391

Others

Related Materials

Natarajan A, Du W, Xiong CY, DeNardo GL, DeNardo SJ and Gervay-Hague J. 2007. Construction of di-scFv through a trivalent alkyne-azide 1,3-dipolar cycloaddition. Chem Commun (Camb). (7):695-7. Epub 2006 Nov 28.

Related Technologies

• High-Throughput Screening of Neuraminidase Inhibitors
• Synthesis of Immunopotent Alpha Glycolipids via Glycosyl Iodides

Additional Technologies by these Inventors

• Synthesis of Immunopotent Alpha Glycolipids via Glycosyl Iodides
• High-Throughput Screening of Neuraminidase Inhibitors
• Novel method to Efficiently Synthesize complex Carbohydrates

Tech ID/UC Case

11289/2006-340-0

Related Cases

2006-340-0

*Abstract

Construction of Multivalent Antibody scFv Through Cu(I) Catalyzed 1,3-Dipolar Cycloaddition

*IP Issue Date

Feb 3, 2015

*Principal Investigator

Name: Sally DeNardo

Department:

Name: Wenjun Du

Department:

Name: Jacquelyn Gervay-Hague

Department:

Name: Arutselvan Natarajan

Department:

Country/Region

USA