A novel approach to engineer proteins with improved stability
- Summary
- Researchers at Oklahoma State University have uncovered a novelmethod to engineer more stable protein variants. Based on a new set ofprinciples, this method is superior compared to previous methods, allowingresearchers to obtain very stable protein variants with ease. OSU researchershave already used this novel set of principles as a guide to introducemutations into a protein, which successfully engineered a protein variant withsubstantially improved stability.
- Technology Benefits
- · Novel method to generate stabileprotein variants · More efficient and robustthan previous methods· Standard, established proceduresto implement method
- Technology Application
- · Generatingproteins and enzymes resistant to heat, acid and alkaline conditions· Production ofenzymes for synthesis of amino acids · Generatingproteases for food processing, bioremediation and detergents· Bioconversionof starch, cellulose, chitin, pulp, proteins and fats. · Production ofbiofuels
- Detailed Technology Description
- None
- *Abstract
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None
- *Background
- For many biotechnological applications it is important to obtainproteins with high stability. Recently, engineering of more stable proteinvariants has attracted considerable attention. Prior work has concentrated onengineering more stable mutants of a protein based on the crystal structure asa starting point and/or to increase the value of free energy between theunfolded and folded state. Unfortunately, these approaches have been met withonly limited success.
- *Stage of Development
- Proof-of-Concept
- Country/Region
- USA
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