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Reverse genetics system for generating RNA viruses and vaccines (SJ-00-0006)

*Abstract

An 8 plasmid system has been developed for generating RNA viruses from cloned DNA. This system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. This system may be used to quickly and easily generate an attenuated version of any RNA virus. 

Key words: RNA viruses, reverse genetics, 8 plasmid system

Granted patents or published applications:  U.S. Patent Nos. 6,951,754; 7,312,064, 7,972,843 and International Publication No WO 01/83794

Related scientific references:  Hoffmann E, et al., "Eight-plasmid system for rapid generation of influenza virus vaccines" Vaccine 20(25-26):3165-3170 (2002).

Hoffmann E, et al., "Unidirectional RNA polymerase I-polymerase II transcription system for the generation of influenza A virus from eight plasmids" J Gen Virol 81(12):2843-2847 (2000).

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USA

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