Abasic Site or Vacant Site-Containing DNAzyme and Aptamer for Label-Free-Fluorescent Detection of Pb2+ and Adenosine with High Sensitivity, Selectivity and Tunable Dynamic Range
- Detailed Technology Description
- A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described.
- Countries
- United States
- Application No.
- 8933210
- *Abstract
-
A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described. In one example, a method for designing label-free fluorescent functional nucleic acid sensors (e.g., those that include a DNAzyme, aptamer or aptazyme) that have a tunable dynamic range through the introduction of an abasic site (e.g., dSpacer) or a vacant site into the functional nucleic acids. Also provided is a general method for designing label-free fluorescent aptamer sensors based on the regulation of malachite green (MG) fluorescence. A general method for designing label-free fluorescent catalytic and molecular beacons (CAMBs) is also provided. The methods demonstrated here can be used to design many other label-free fluorescent sensors to detect a wide range of analytes. Sensors and methods of using the disclosed sensors are also provided.
For more information about this technology, please contact the University of Illinois at Urbana-Champaign Office of Technology Management at otm@illinois.edu.
- *IP Issue Date
- None
- *IP Type
- Utility
- Country/Region
- USA
