PRTA Gene And Uses Therefor
- Detailed Technology Description
- This invention relates to biocontrol of plant pathogens. More particularly, this invention relates to the regulation of microbial synthesis of metabolites that interfere with plant pathogen proliferation and/or their infection of plants. Canola is one of the major oilseed crops grown worldwide. Sclerotinia sclerotiorum is a fungus that causes devasting losses in canola as well as over 400 other crop species. A bacterium isolated from soybean root tips, called Pseudomonas chlororaphis strain PA23, was found to protect canola from the disease caused by this fungus. Pseudomonas chlororphis strain PA23 was isolated from soybean root tips and has demonstrated excellent ability to protect canola from stem rot disease caused by the fungus Sclerotinia sclerotiorum; under field as well as greenhouse conditions. Consequently, the PA23 bacterium represents an attractive alternative to chemical pesticides for managing diseases that threaten canola.
- Supplementary Information
- Patent Number: WO2008049230A1
Application Number: WO2007CA1913A
Inventor: DE KIEVIT, Teresa | FERNANDO, Dilantha
Priority Date: 24 Oct 2006
Priority Number: WO2008049230A1
Application Date: 24 Oct 2007
Publication Date: 2 May 2008
IPC Current: C12N001531 | A01N006302 | A01P000300 | C07K001421 | C12N000121
Assignee Applicant: University of Manitoba
Title: PTRA GENE AND USES THEREFOR | GÈNE PTRA ET SES UTILISATIONS
Usefulness: PTRA GENE AND USES THEREFOR | GÈNE PTRA ET SES UTILISATIONS
Summary: The nucleic acid, microbial cells, and composition are useful for inhibiting Sclerotinia infections of Brassica plants (all claimed).
Novelty: New isolated Pseudomonas chlororaphis LysR-type putative transcriptional regulator (ptrA) gene encodes a protein inhibitory to Sclerotinia, useful for inhibiting Sclerotinia infections of Brassica plants
- Industry
- Disease Diagnostic/Treatment
- Sub Category
- Cancer/Tumor
- *Abstract
-
Disclosed herein is the characterization of a P. chlororaphis strain PA23 derivative with a mutation in a lysR-type transcriptional regulator, designated PtrA. Unlike the wild type, the ptrA-deficient strain (PA23-443) was unable to protect canola from Sclerotinia stem rot in greenhouse assays, highlighting the importance of PtrA in the biocontrol function of P. chlororaphis strain PA23. Genetically modified strains of PA23, containing an essential regulatory gene (ptrA) under control of a consitutively expressed promoter, will exhibit superior disease protection under a wide range of environmental conditions. Since PA23 produces pyrrolnitrin, which is active against a broad range of fungal pathogens, PA23-based biopesticides have large commerical promise.
In this disclosure, improved versions of P. chlororaphis strain PA23 that produce antifungal products at the highest level possible, regardless of environmental conditions are described. These versions were achieved by placing the essential antifungal genes in the “ON” position all of the time, so that the required products are always made regardless of environmental conditions.
This research resulted in the creation of an improved biocontrol bacterium capable of protecting canola from the disease. This disclosure herein describes the methods pertaining to the isolation of an nucleic acid molecule comprising a nucleotide sequence set forth in SEQ ID NO:1, with the nucleotide sequence encoding a protein inhibitory to Sclerotinia spp., with the protein comprising an amino acid sequence set forth in SEQ ID No:2. This disclosure describes a nucleotide construct comprising a nucleic acid molecule operably linked to a suitable promoter, which may be stably incorporated into the genome of a selected cell for expression of the nucleotide sequence therein. Suitable cells are exemplified by Pseudomonas sp. Compositions comprising microbial cells comprising the nucleotide sequence set forth in SEQ ID NO: 1, may be applied to Brassica seeds or alternatively, to developing Brassica plants to inhibit Sclerotinia infections thereof and therein.
- *Inquiry
- Jody Dexter,Ph.DTechnology ManagerPh:+1(204)474.8966Email: jody.dexter@umanitoba.ca
- Country/Region
- USA
