Increased Enzyme Production via Pretreatments
Researchers at Purdue University have developed technology that utilizes liquefaction of pretreated and sterilized sugarcane bagasse for enhancing endoglucanase production through SmF by Aspergillus niger (A. niger). After initial SSF of steam pretreated bagasse solids by A. niger, fed-batch addition of the substrate to cellulase in buffer and reaction, resulted in a liquid slurry with a viscosity of 0.30 ± 0.07 Pa•s at 30% (w/v) solids. The addition of A. niger for submerged fermentation of sterile liquefied bagasse at 23% (w/v) solids resulted in approximately 15 times higher productivity than SSF of non-liquefied bagasse. These results demonstrate the utility of liquefied bagasse as a culture medium for enzyme production in submerged fermentations.
Reduced power input required for mixing the slurry Liquefied biomass gives a 15 times higher enzyme yield compared to solid-state fermentation Enhancement of the enzyme yield
Chemical analysis companiesChemical analysis laboratories
Michael LadischPurdue Agricultural and Biological EngineeringLORRE Laboratory of Renewable Resources Engineering
United States
N/A
USA
