Improved Transformation of Clostridium
- Technology Benefits
- Simpler andfaster compared to current alternatives
- Detailed Technology Description
- Method for methylation of DNA to ensure transformation of Clostridium acetobutylicum #researchtool #reagent
- *Abstract
-
Northwestern researchers have designed a plasmid that expresses a methyltransferase from Bacillus subtilis in Escherichia coli. This plasmid, pAN1, can be co-expressed with genes from C. acetobutylicum to methylate them, protecting them against degradation by C. acetobutylicum nucleases upon transformation. C. acetobutylicum is a well-studied solventogenic bacterium with many industrial applications. However, it expresses many nucleases that interfere with introduction of plasmids, so it has been difficult to generate genetic mutants or deletions that could improve solvent production by this bacterium. Thus, this innovative reagent from the Papoutsakis laboratory will quicken the pace of basic genetic research of C. acetobutylicum with potentially significant implications for solvent production by this bacterium.
- *Inventors
- Lee MermelsteinEleftherios Papoutsakis*
- *Publications
- Mermelstein LD,Papoutsakis ET (1993) In Vivo Methylationin Escherichia coli by the Bacillus subtilis Phage ϕ3TI Methyltransferase to Protect Plasmids from Restriction upon Transformation ofClostridium acetobutylicum ATCC824, Applied and Environmental Microbiology,59: 1077-1081.
- Country/Region
- USA
