Novel Liposomes with Improved Transfection Efficiency on Human Umbilical Artery Endothelial Cells
- Technology Benefits
- • Enhanced transfection efficiency (up to a 30-fold increase) • Customizable to various cell types and conditions including the presence of serum
- Detailed Technology Description
- Effective method to transfect human vascular endothelial cells.
- *Abstract
-
Northwestern researchers have developed a new method to transfect DNA into vascular endothelial cells that is up to 30 times more effective than commercially available products. The novel transfection agent, MLS, is a mixture of two cationic components that are combined in different proportions to optimize the efficiency of DNA delivery. The agent dramatically improves transfection efficiency when compared with even Qiagen's Effectene®, which is among the more effective agents for "difficult-to-transfect" cells such as smooth muscle cells, primary cell lines, and endothelial cells. These novel compounds may simply be used by laboratories for such cells for increased gene expression. Alternatively, because of the success of this agent in transfecting HUAEC, scientists are now able to explore the use of HUAEC as a viable gene therapy target. Furthermore, this research tool offers new possibilities for optimizing in vivo gene delivery. The significance of this agent is its markedly enhanced transfection efficiency which can be optimized to a given cell type and conditions (in vitro vs. in vivo).
- *Inventors
- Li Wang Robert MacDonald
- *Publications
- Wang L and MacDonald R (2004) Improved Transfection with Mixtures of Cationic Lipids: Application to Human Umbilical Artery Endothelial Cells (HUAEC). Molecular Therapy. 9: S178. Wang L and MacDonald R (2004) New strategy for transfection: mixtures of medium-chain and long-chain cationic lipids synergistically enhance transfection. Gene Therapy. 11: 1358-1362. Koynova R, Tarahovsky YS, Wang L, MacDonald RC (2007) Lipoplex formulation of superior efficacy exhibits high surface activity and fusogenicity, and readily releases DNA. Biochimica et Biophysica Acta. 1768: 375-386.
- Country/Region
- USA
