A Function Blocking Monoclonal Antibody Against alpha4 Laminin
- Detailed Technology Description
- Researchers at Northwestern University have developed a function blocking monoclonal antibody (mAb) to thea4 subunit of laminin. #therapeutics #cancer #antibody #researchtool
- *Abstract
-
This antibody, mAb 2A3, inhibits angiogenesis and endothelializationin vitro and in vivo. Integrins are cellular receptors that mediate cell-cell and cell-extracellular matrix interactions, and integrin avß3 is postulated to play a major role in angiogenesis by acting as a receptor fora subunits and modulating a variety of cell-cell interactions. Thea4 subunit is a ligand for theavß3 integrin, and the 2A3 antibody acts to blocka4 subunit interactions withavß3
In vivo matrigel assays have demonstrated that the 2A3 antibody can inhibit angiogenesis. Experiments have demonstrated that the 2A3 antibody is able to inhibitin vitro wound healing of endothelial cells. Currently, an antibody toavß3 integrin (Vitaxin) is being evaluated in clinical trials. However, data obtained by our investigators indicate that the 2A3 antibody developed at Northwestern is more efficient than Vitaxin at blocking two essential aspects of angiogenesis: branching morphogenesis and migration of endothelial cells.
- *Inventors
- Meredith Gonzales Jonathan Jones*
- *Publications
- Gonzales, M. et al., Molecular Biology of the Cell 12: 85-100, January 2001 Gonzalez, AM et al., PNAS 99 (25): 16075-16080, December 10, 2002
- Country/Region
- USA
