TEX101-Driven Expression of Improved CRE Recombinase (iCRE) in Transgenic Mice (14005)
- *Abstract
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Features and Benefits
- Creation of a transgenic mouse line expressing improved Cre Recombinase (iCRE) in spermatogenic cells of adult mice.
- Allows inactivation of a floxed gene in spematogenic cells.
- Provides excellent tool to understand the physiological roles of male germ cell-expressing genes involved in normal spermatogenesis and male fertility.
*This Technology is available for licensing, further development, or industrial partnering*
Technology
Researchers at the University of Louisville have developed a line of transgenic mice expressing improved Cre recombinase (iCre) driven by the mouse Tex101 promoter. iCre recombinase is expressed in the adult testes of the resulting Tex101-iCre mice, but not in heart, liver, spleen, lung, adrenal gland, pancreas, thymus, pituitary, ovary, oviduct or uterus. iCre recombinase activity was detected in the prespermatogonia within the testis from eight-day old mice; however, Cre activity was weak in spermatogonia and interstitial cells of adult mice. Robust Cre activity was detected in spermatocytes and spermatids within seminiferous tubules of adult testes. As a test of the use of these mice, breeding wild type females with homozygous floxed fibroblast growth factor receptor 2 (Fgfr2) males carrying the Tex101-iCre transgene did not produce any progeny with the floxed Fgfr2 allele. This result demonstrates that the complete deletion of the floxed Fgfr2 allele can be achieved in the male germline using Tex101-iCre mice. Also, FGFR 2 proteins were not detected in spermatocytes and spermatids of adult Fgfr2fl/fl: Tex101iCre mice.
In sum, the UofL's Tex101-iCre mouse line allows the inactivation of a floxed gene in spermatogenic cells (e.g. spermatocytes and spermatids) in resulting adult mouse models.
Markets Addressed
Deletion of genes in the spermatogenic cells within the testes using UofL's new Tex101-iCre mice with the Cre/loxP technology provides an excellent tool to understand the physiological roles of male germ cell-expressing genes involved in normal spermatogenesis and male fertility.
Technology Status
- IP Status: Research Tool
- Fields of Use Available: Non-exclusive Commercial and Research Uses.
- Country/Region
- USA
