Caged Nucleotides
- Detailed Technology Description
- None
- *Abstract
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A family of photolytically caged nucleotides, including cyclic nucleotides with calcium ion mobilizing agents and antagonists, has been developed. The compounds are useful for the photolytic generation of free nucleotides in aqueous samples, for example, in the study of calcium mobilization in cells and cell homogenates.The uncaging efficiency of the cyclic nucleotides has been found to be better than that of other caged compounds. Often Ca2+ leakage can occur during microinjection, causing the possibility of being mistaken as Ca2+ released by cyclic ADP ribose (cADPR). These new caged nucleotides may provide a possible alternative approach to introducing cyclic nucleotides into cells other than the current method of microinjection. With caged nucleotides, Ca2+ release occurs through induction by ultraviolet photolysis, thus eliminating possible injection artifact. The focused laser illumination allows for free nucleotides to be generated at specific locations within a single cell, either outside the cell or in a cell-free medium.
- *Principal Investigator
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Name: Hon Cheung Lee
Department:
Name: Timothy Walseth, Professor
Department: Pharmacology
- Country/Region
- USA
