Cellular Imaging Probe Determination
- Technology Benefits
- Highly sensitive biological and cellular pH monitoring Increased resolution allows 3D imaging of cells and biological samples Low toxicity
- Technology Application
- Bio Imaging, Bio-Sensors, Research & Development
- Detailed Technology Description
- Scientists at UCF have synthesized several fluorescent probes which make use of two photon absorption to monitor pH in biological systems as well as for 3D imaging of cells. These two photon absorbing probes have several advantages: less scattering from cells, deeper penetration of light in samples and the use of near-infrared radiation for excitation. 3D imaging is improved over single photon absorption methods due to reduced scattering and deeper cell penetration. These probes are water soluble and highly effective as an intracellular pH indicator. Intracellular pH is determined by the ratio of fluorescence emission from the probe at two specific wavelengths. The dye has tested to have good permeation into the cell and disperses quickly once inside. Cell toxicity of the probe has been monitored and is extremely low over a wide concentration range.
- Supplementary Information
- Inventor: Albert, Matthew L. | Bhardwaj, Nina | Steinman, Ralph M. | Inaba, Kayo | Darnell, Robert
Priority Number: US6602709B1
IPC Current: A61K003512 | A61K003526 | A61K003900 | A61K0039145 | A61K0039385 | A61P003100 | A61P003500 | C12N000502 | C12N00050784 | A61K004800
US Class: 435372 | 4241841 | 4242771 | 4242781 | 435002 | 435366 | 4353721 | 435373
Assignee Applicant: The Rockefeller University,New York
Title: Methods for use of apoptotic cells to deliver antigen to dendritic cells for induction or tolerization of T cells
Usefulness: Methods for use of apoptotic cells to deliver antigen to dendritic cells for induction or tolerization of T cells
Summary: The DCs can be loaded with antigens such as antigens derived from influenza virus, malaria, HIV, EBV, human papilloma virus, CMV, renal cell carcinoma antigens, and melanoma antigens. In addition, self antigens that are targets of autoimmune responses or other antigens for which it is desired to attenuate an immune response can be expressed on donor cells using the methods, e.g. insulin, histones or GAD. The products can be used for the prophylactic or therapeutic treatment of diseases such as e.g. bacterial infections, protozoan infections, such as malaria, listeriosis, microbial infections, viral infections such as HIV or influenza, cancers or malignancies such as melanoma, autoimmune diseases such as psoriasis and ankolysing spondylitis.
Novelty: Production of antigen loaded dendritic cells, used for developing products for treating e.g. cancer or malignancies, microbial infections or autoimmune diseases
- Industry
- Disease Diagnostic/Treatment
- Sub Category
- HIV
- *Abstract
-
Monitoring intracellular pH is crucial for understanding the regulation mechanism of many physiological functions, such as: how cells work, biological mechanisms and drug reactions. Understanding these functions is important for curing diseases, improving medicines and the general health and well being of people. Optical methods of determining pH have many advantages over currently available chemical methods, including: rapid response time, being noninvasive, high signal-to-noise ratio and sensitivity. Fluorescent pH indicators that make use of single photon absorption have several limitations: interference or scattering from biological fluids and tissue, photodamage of samples and difficulty in analyzing inter and intracellular pH differences.
- *Principal Investigator
-
Name: Dr. Kevin Belfield
Department:
- Country/Region
- USA
