Optical Alignment Deformation Spectroscopy
- Technology Benefits
- Main Advantages of this InventionΓÇóHigh through-put assay measuring individual cells instead of population averageΓÇóReagent and label freeΓÇóMore accurate than current methods
- Technology Application
- Potential Areas of Application ΓÇóDisease screeningΓÇóHigh through-put blood testing method
- Detailed Technology Description
- None
- Industry
- Optics
- Sub Category
- Optical Fiber
- *Abstract
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Cell mechanical properties are a useful measure of phenotype that can be quantified by cell deformability. There is a lack of high-throughput methods to investigate the mechanical properties of large populations of individual cells. To address this need, Mines has developed optical alignment deformation spectroscopy (OADS), a technique where hydrodynamic interactions between individual cells are used to create deformation. In OADS, a linear optical trap is used to align two incoming cells in a microfluidic cross-flow geometry, allowing hydrodynamic forces to induce a collision between cells at the stagnation point. After the interaction, the cells leave the stagnation point and a new pair of cells enters the trap. A convenient model cell to characterize OADS is the human erythrocyte because of its well-known mechanical properties. Deformation data of erythrocytes is fit to a linear viscoelastic constitutive model (Voigt). The results show OADS has potential as an accurate high-throughput individual cell mechanical cytometer.
- *Principal Investigator
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Name: David Marr
Department:
Name: Keith Neeves
Department:
Name: Kevin Roth
Department:
- Country/Region
- USA
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