A-25 Method for Measuring Polyamines in Erythrocytes
- Technology Benefits
- Measuring polyamines in blood instead of urine is easy and accurate: the existing method for measuring polyamine in urine requires pretreatment for hydrolysis.polyamine concentrations in urine do not always correlate directly to concentrations within the body. Improved simplicity: Some enzymatic methods use two types of amine oxidases derived from bacteria in two-step enzymatic reactions.
- Technology Application
- * Measuring polyamines, spermine and spermidine, in erythrocytes* Diagnostic methods* Diagnostic kit
- Detailed Technology Description
- None
- Supplementary Information
- Patent Number: US7208284B2
Application Number: US20043416A
Inventor: Takagi, Kenji
Priority Date: 9 Jun 2004
Priority Number: US7208284B2
Application Date: 6 Dec 2004
Publication Date: 24 Apr 2007
IPC Current: G01N002178 | G01N003300 | C12Q000102 | C12Q000126 | G01N0033574
US Class: 43500725
Assignee Applicant: National University Corporation Nagoya University,Nagoya shi
Title: Method for measuring polyamines in erythrocytes, diagnostic method and kit for measuring polyamines in erythrocytes
Usefulness: Method for measuring polyamines in erythrocytes, diagnostic method and kit for measuring polyamines in erythrocytes
Summary: The invention deals with a method for measuring polyamines in erythrocytes (blood). The method is useful for diagnosis of diseases (diagnosis of infections, cancer, cirrhosis, diabetes (type I), Alzheimer's disease, diagnosis for prognosis of organ excision or organ transplantation). (All claimed). The method is useful for measuring (I) in urine.
Novelty: Measuring polyamines in erythrocytes comprises reacting eluate of erythrocytes with polyamine oxidase having substrate specificity to spermine and spermidine and determining hydrogen peroxide formed with sensitive chromogen
- Industry
- Disease Diagnostic/Treatment
- Sub Category
- Cancer/Tumor
- *Abstract
-
This novel method for measuring polyamines in erythrocytes consists of (1) separating and purifying an eluate of erythrocytes from blood, (2) reacting the eluate with polyamine oxidase having a substrate specificity to the polyamineΓÇÖs spermine and spermidine, (3) determining the amount of hydrogen peroxide formed in (2) with a highly sensitive chromogen, and (4) measuring the spermine and spermidine in erythrocytes.
- Country/Region
- USA
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