A-25 Method for Measuring Polyamines in Erythrocytes
- Technology Benefits
- Measuring polyamines in blood instead of urine is easy and accurate: the existing method for measuring polyamine in urine requires pretreatment for hydrolysis.polyamine concentrations in urine do not always correlate directly to concentrations within the body. Improved simplicity: Some enzymatic methods use two types of amine oxidases derived from bacteria in two-step enzymatic reactions.
- Technology Application
- * Measuring polyamines, spermine and spermidine, in erythrocytes* Diagnostic methods* Diagnostic kit
- Detailed Technology Description
- None
- Industry
- Disease Diagnostic/Treatment
- Sub Category
- Cancer/Tumor
- *Abstract
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This novel method for measuring polyamines in erythrocytes consists of (1) separating and purifying an eluate of erythrocytes from blood, (2) reacting the eluate with polyamine oxidase having a substrate specificity to the polyamineΓÇÖs spermine and spermidine, (3) determining the amount of hydrogen peroxide formed in (2) with a highly sensitive chromogen, and (4) measuring the spermine and spermidine in erythrocytes.
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