一种流式荧光显微成像装置及方法
- Summary
- 本发明为一种流式荧光显微成像装置，能够解决成像流式细胞仪的焦深小及对运动物体成像时的拖尾问题。
- Technology Benefits
- 本发明的有益效果包括：1)采用接近衍射极限厚度的片光源激发细胞的自发荧光或非自发荧光，可以克服荧光显微成像焦深小的问题；2)仅有处在焦深范围内的细胞被激发，发射荧光，可以有效的降低荧光像的背景，因此能够有效的提高系统的灵敏度；3)细胞垂直通过激发光源，采用成像方向与样品流动方向平行的方式对细胞进行荧光成像，可以很好地抑制拖尾现象；4)该装置可以允许多个细胞同时被检测，从而可以提高系统的检测速度。
- Technology Application
- 本发明可用於生物学与医学领域上的 成像流式细胞仪
- Detailed Technology Description
- 一种流式荧光显微成像装置，包括光源、液体样品进样单元和荧光显微成像单元，其特征在于所述光源为激光片光源，液体样品的流动方向与所述荧光显微成像单元的光轴相互平行，与激光片光源激发单元的光轴相互垂直。
- Supplementary Information
- Patent Number: CN103308440A
Application Number: CN201310202769A
Inventor: LI, Jian-ping | CHEN, Qi-yao | WU, Jiang-lai
Priority Date: 28 May 2013
Priority Number: CN103308440A
Application Date: 28 May 2013
Publication Date: 18 Sep 2013
IPC Current: G01N001514 | G01N002164
Assignee Applicant: Shenzhen Institute Of Hong Kong Baptist University
Title: Flow type fluorescence microscopy imaging device and method | A kind of flow fluorescent micro-imaging device and method
Usefulness: Flow type fluorescence microscopy imaging device and method | A kind of flow fluorescent micro-imaging device and method
Novelty: Fluorescent micro-imaging device has excitation unit that is arranged vertically to optical axis of light source
- Industry
- Measurement/Testing
- Sub Category
- Measurement Tool
- Application Date
- 2013.05.28
- Application No.
- 201310202769
- Others
- Filed
- ID No.
- G02/RC/Fluo/CN01
- Country/Region
- Hong Kong
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